诱导性协同共刺激分子及程序性细胞死亡受体1在大疱性类天疱疮皮损中的表达及意义  

Expression of inducible costimulator and programmed cell death 1 receptor in skin lesions of bullous

作　　者：杨敏[1] 朱英华[1] 彭琳琳[1] 王舒靖[1] 靳亚莉[1] Yang Min;Zhu Yinghua;Peng Linlin;Wang Shujing;Jin Yali(Department of Dermatology,Dalian Dermatosis Hospital,Dalian 116021,China)

机构地区：[1]大连市皮肤病医院皮肤科,116021

出　　处：《中华皮肤科杂志》2020年第10期813-815,共3页Chinese Journal of Dermatology

基　　金：大连市卫生健康委科研项目。

摘　　要：目的通过检测滤泡辅助性T细胞(Tfh)相关分子诱导性共刺激分子(ICOS)及程序性细胞死亡受体1(PD-1)在大疱性类天疱疮(BP)患者皮损中的表达,探讨Tfh在BP发病机制中的作用。方法收集大连市皮肤病医院2014-2017年间确诊的BP患者石蜡组织标本21份,其中女7例,男14例,平均年龄72.57岁。应用免疫组化SP法检测BP皮损中ICOS与PD-1的表达,以10例正常皮肤组织作为对照组。结果 ICOS、PD-1在BP皮损中均主要表达于表皮基底层、棘细胞层、颗粒层、角质层,以棘细胞层表达最为显著,细胞质、细胞核均见表达,偶见细胞膜表达,真皮层炎症细胞中亦有表达;正常皮肤组织中ICOS、PD-1表达少见。BP组ICOS表达率为85.71%(18/21),PD-1表达率为47.62%(10/21),均高于正常对照组(均为0),差异有统计学意义(P值分别<0.001、<0.05)。结论 Tfh细胞相关分子ICOS、PD-1在BP发病机制中可能发挥重要作用。Objective To determine the expression of T follicular helper(Tfh)cell?related molecules inducible costimulator(ICOS)and programmed cell death 1 receptor(PD?1)in skin lesions of patients with bullous pemphigoid(BP),and to explore the role of Tfh cells in the pathogenesis of BP.Methods???Twenty?one paraffin?embedded tissue specimens were collected from 21 patients with confirmed BP in Dalian Dermatosis Hospital from 2014 to 2017,including 7 females and 14 males with an average age of 72.57 years.Ten normal skin tissues served as control group.Immunohistochemical SP method was used to determine the expression of ICOS and PD?1 in BP skin lesions and normal skin tissues.Results In BP lesions,ICOS and PD?1 were mainly expressed in the basal layer,spinous layer,granular layer and stratum corneum,especially in the spinous layer.Additionally,they were both expressed in the cytoplasm and nuclei,occasionally expressed on the cell membrane,and also expressed in inflammatory cells in the dermis.However,ICOS and PD?1 were rarely expressed in the normal skin tissues.The expression rates of ICOS and PD?1 were significantly higher in the BP group(85.71%[18/21],47.62%[10/21],respectively)than in the normal control group(both were 0;P<0.001,<0.05,respectively).Conclusion Tfh cell?related molecules ICOS and PD?1 may play important roles in the pathogenesis of BP.

关 键 词：类天疱疮 大疱性 程序性细胞死亡受体1 T淋巴细胞 辅助诱导 诱导性共刺激分子 滤泡辅助性T细胞 

分 类 号：R758.66[医药卫生—皮肤病学与性病学]