竹黄菌CGMCC 2201生产竹红菌素发酵培养基的优化  

Optimization of fermentation media for hypocrellin production by Shiraia bambusicola CGMCC 2201

作　　者：卜光明 杨海龙[2] Bu Guang-ming;Yang Hai-long(Zhejiang Ruibang Pharmaceutical Co.,Ltd.,Wenzhou 325000;School of Life&Environmental Science,Wenzhou University,Wenzhou 325035)

机构地区：[1]浙江瑞邦药业股份有限公司,温州325000 [2]温州大学生命与环境科学学院,温州325035

出　　处：《中国抗生素杂志》2020年第7期660-665,共6页Chinese Journal of Antibiotics

摘　　要：目的优化竹黄菌CGMCC 2201发酵培养基提高竹红菌素产量。方法采用单因素试验确定培养基关键因素,利用中心组合设计与响应面分析法获得关键因素的最佳浓度,结合植物油添加试验,获得最优发酵培养基配方。结果葡萄糖、硫酸铵、磷酸二氢钾、硫酸镁是发酵培养基中的关键因素。最优发酵培养基配方(g/L):葡萄糖47.33,硫酸铵2.14,磷酸二氢钾2.87,硫酸镁1.68,豆油10。采用此发酵培养基的竹红菌素产量达257.66mg/L,与优化前培养基相比,提高了141.25%。结论培养基是影响竹黄菌CGMCC 2201生物合成竹红菌素的重要因素,发酵培养基优化后显著提高了竹红菌素的发酵产量。Objective To enhance the hypocrellin production by optimizing the fermentation media of Shiraia bambusicola CGMCC 2201. Methods The key components of fermentation media were firstly determined by onefactor-at-a-time experiment, and then the concentrations of key components were optimized by the central composite design and the response surface analysis. Moreover, the effects of plant oils on the hypocrellin biosynthesis were also investigated to obtain the optimal fermentation medium formula for hypocrellin production by submerged culture of S. bambusicola CGMCC 2201. Results Four key components of fermentation media were selected, which were glucose, ammonium sulfate, potassium dihydrogen phosphate, and magnesium sulfate, and soybean oil significantly enhanced the biosynthesis of hypocrellin. The components of the optimized fermentation media(g/L) were: glucose 47.33, ammonium sulfate 2.14, potassium dihydrogen phosphate 2.87, magnesium sulfate 1.68, and soybean oil 10. The hypocrellin yield reached 257.66 mg/L in the optimal media, 141.25% higher than that in the initial media. Conclusion Media components were the important factors in the biosynthesis of hypocrellin in S. bambusicola CGMCC 2201, and the hypocrellin production was significantly increased by the optimization of fermentation media.

关 键 词：竹黄菌 竹红菌素 发酵培养基 优化 响应面法 

分 类 号：Q815[生物学—生物工程]