HPLC-DAD法同时测定人参再造丸中11个成分的含量  

Simultaneous determination of eleven components in Renshenzaizao pills by HPLC-DAD

作　　者：张博 刘惠军 田兰 王彦[2] 陈睿 闫超[2] ZHANG Bo;LIU Hui-jun;TIAN Lan;WANG Yan;CHEN Rui;YAN Chao(Shanghai Songjiang Institute for Food and Drug Control,Shanghai 201600,China;School of Pharmacy,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海市松江食品药品检验所,上海201600 [2]上海交通大学药学院,上海200240

出　　处：《实用药物与临床》2020年第10期913-920,共8页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立同时测定人参再造丸中葛根素、芍药苷、毛蕊异黄酮葡萄糖苷、甘草苷、2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡萄糖苷、5-O-甲基维斯阿米醇苷、柚皮苷、橙皮苷、人参皂苷Rg 1、人参皂苷Re、哈巴俄苷含量的高效液相色谱方法。方法采用Merck Purospher STAR C 18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.05%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 ml/min,柱温35℃,检测波长为203 nm(人参皂苷Rg 1、人参皂苷Re)、237 nm(芍药苷、5-O-甲基维斯阿米醇苷)、254 nm(葛根素、毛蕊异黄酮葡萄糖苷)、280 nm(甘草苷、柚皮苷、橙皮苷、哈巴俄苷)、320 nm(2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡萄糖苷)。结果各待测组分分离度良好;葛根素、芍药苷、毛蕊异黄酮葡萄糖苷、甘草苷、2,3,5,4′-四羟基二苯乙烯-2-O-β-D葡萄糖苷、5-O-甲基维斯阿米醇苷、柚皮苷、橙皮苷、人参皂苷Rg 1、人参皂苷Re、哈巴俄苷11个成分在相应的范围内均呈良好的线性关系,相关系数(r)均不低于0.9991,平均回收率(n=9)分别为97.8%、92.0%、96.5%、97.0%、87.1%、95.6%、95.3%、95.1%、95.7%、94.6%、97.9%。结论该方法经方法学验证可用于人参再造丸的质量控制。Objective To develop an HPLC method for simultaneous determination of puerarin,paeoniflorin,calycosin7-O-β-D-glucopyranoside,liquiritin,2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside,5-O-methylvisammioside,naringin,hesperidin,ginsenoside Rg 1,ginsenoside Re and harpagoside in Renshenzaizao pills.Methods The samples were separated on a Merck ODS C 18 column(4.6 mm×250 mm,5μm)by a gradient elution using acetonitrile(A)and 0.05%phosphoric acid aq.(B)as mobile phase at the flow rate of 1.0 ml/min,and the column temperature was 35℃.The eluate was detected by multiple wavelength:203 nm for ginsenoside Rg 1 and ginsenoside Re,237 nm for paeoniflorin and 5-O-methylvisammioside,254 nm for puerarin and calycosin7-O-β-D-glucopyranoside,280 nm for liquiritin,naringin,hesperidin and harpagoside,and 320 nm for 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside.Results Excellent chromatographic separation was achieved with good linearity(r≥0.9991)of puerarin,paeoniflorin,calycosin7-O-β-D-glucopyranoside,liquiritin,2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside,5-O-methylvisammioside,naringin,hesperidin,ginsenoside Rg 1,ginsenoside Re and harpagoside within their corresponding linear ranges.The average recoveries(n=9)were 97.8%,92.0%,96.5%,97.0%,87.1%,95.6%,95.3%,95.1%,95.7%,94.6%and 97.9%,respectively.Conclusion The established method is validated by methodology,and is suitable for the quality control of Renshenzaizao pills.

关 键 词：人参再造丸 多组分含量测定 高效液相色谱 

分 类 号：R286.0[医药卫生—中药学]