苍术挥发油对溃疡性结肠炎大鼠的改善作用  

The improving effect of volatile oil of rhizoma atractylodis on ulcerative colitis in rats

作　　者：刘晓兰[1] 张永忠[1] 张俊玲 聂晓博[1] 贾琳[3] 刘会丽[1] LIU Xiao-lan;ZHANG Yong-zhong;ZHANG Jun-ling;NIE Xiao-bo;JIA Lin;LIU Hui-li(Shijiazhuang Medical College,Shijiazhuang 050599,China;Caofeidian Vocational and Technical College;Hebei Hospital of Traditional Chinese Medicine)

机构地区：[1]石家庄医学高等专科学校,050599 [2]曹妃甸职业技术学院 [3]河北省中医院

出　　处：《天津医药》2020年第10期956-960,共5页Tianjin Medical Journal

基　　金：河北省中医药管理局计划项目(2019503)。

摘　　要：目的研究苍术挥发油对溃疡性结肠炎(UC)模型大鼠的改善作用。方法以5%的2,4,6-三硝基苯磺酸灌肠建立UC大鼠模型。将模型大鼠按照随机数字表法分为模型组,阳性对照组(美沙拉嗪0.36 g/kg灌胃),低、中、高剂量苍术挥发油组(分别以1、2、4 g/kg苍术挥发油灌胃),每组10只。另设空白对照组大鼠10只(灌胃等体积蒸馏水)。各组大鼠每日给药(或蒸馏水)1次,连续10 d。记录各组大鼠模型制备前、给药前和末次给药后的体质量变化。末次给药后,取大鼠结肠组织,HE染色观察结肠病理改变,酶联免疫吸附测定(ELISA)法检测白细胞介素(IL)-6和肿瘤坏死因子(TNF)-α含量;实时荧光定量逆转录聚合酶链反应(qPCR)检测组织中自噬基因Beclin 1和P62 mRNA表达,Western blot法检测微管相关蛋白1轻链3(LC3)蛋白表达水平。结果与空白对照组相比,模型组给药后体质量下降,结肠黏膜上皮细胞脱落,隐窝扭曲或萎缩,炎症细胞浸润;结肠组织中IL-6和TNF-α含量升高,Beclin 1和P62 mRNA表达水平升高(P<0.05);LC3Ⅱ/Ⅰ蛋白表达无明显变化(P>0.05)。与模型组相比,中、高剂量组和阳性对照组大鼠给药后体质量升高,结肠上皮细胞损伤减轻,结肠组织IL-6和TNF-α含量下降,LC3Ⅱ/Ⅰ表达升高;与模型组相比,高剂量组和阳性对照组Beclin 1和P62 mRNA表达明显升高(P<0.05)。低剂量组与模型组相比,各指标变化差异无统计学意义(P>0.05)。结论苍术挥发油能够改善UC模型大鼠结肠组织病理损伤,降低结肠组织IL-6、TNF-α含量,可能与上调Beclin1、P62 mRNA表达和LC3Ⅱ/Ⅰ蛋白的表达量有关。Objective To study the effect of volatile oil of rhizoma atractylodis(VRA) on the improvement of ulcerative colitis(UC) in rats.Methods The rat model of UC was established with 5% 2,4,6-trinitrobenzenesulfonic acid enema.The model rats were randomly divided into model group,positive control group(gavage mesalamine 0.36 g/kg),low dose,medial dose and high dose groups(gavage VRA 1,2,4 g/kg respectively),with 10 rats in each group.Another 10 rats were set in the control group(gavage equal volume distilled water).Rats in each group were given the drug(or distilled water) once a day for10 days.The changes of body weights before modeling,before and after administration were recorded in each group.After the last administration,colon tissues of rats were taken,and pathological changes were observed by HE staining.The levels of interleukin 6(IL-6) and tumor necrosis factor-α(TNF-α) in colon tissue were determined by enzyme linked immunosorbent assay(ELISA).Belinl and P62 mRNA expressions were detected by real-time PCR.LC3 protein expressions were detected by Western blot assay.Results Compared with the control group,the model group showed the decreased body mass,shedding of colonic mucosal epithelial cells,distortion or atrophy of crypt,and infiltration of inflammatory cells.The levels of IL-6 and TNF-α in colon tissues were increased,and the mRNA levels of P62 and Beclin 1 were increased(P <0.05).There were no significant differences in the levels of LC3 Ⅱ/Ⅰ protein expressions between control group and model group(P>0.05).Compared with the model group,rats in the medial dose group,high dose group and the positive control group increased in body weight after administration.The levels of IL-6 and TNF-α were significantly decreased.Beclin 1 and P62 mRNA expressions were significantly up-regulated.The mRNA levels of Beclin 1 and P62 were significantly increased in the high dose group and positive control group than those of model group(P <0.05).There were no significant differences in the changes of each index betwe

关 键 词：结肠炎 溃疡性 大鼠 Sprague-Dawley 白细胞介素-6 肿瘤坏死因子-α Beclin-1蛋白 苍术挥发油 P62 微管相关蛋白1轻链3 

分 类 号：R965.1[医药卫生—药理学]