鸡PIK3C2A基因第6内含子多态性及其与体尺和屠体性状的关联分析  

Polymorphisms of PIK3C2A Gene Intron 6 and Their Association with Body Size and Carcass Traits in Chickens

作　　者：杨丕才 刘珂 曹海月 刘虹华 温娅娅 毛海光 尹兆正[2] YANG Picai;LIU Ke;CAO Haiyue;LIU Honghua;WEN Yaya;MAO Haiguang;YIN Zhao-zheng(Institute of Animal Health Supervision of Jingdong YiAutonomous County,Yunnan Jingdong 676200,China;College of Animal Sciences,Zhejiang University,Zhejiang Hangzhou 310058,China)

机构地区：[1]云南省景东彝族自治县动物卫生监督所,云南景东676200 [2]浙江大学动物科学学院,浙江杭州310058

出　　处：《中国畜牧杂志》2020年第9期73-77,83,共6页Chinese Journal of Animal Science

基　　金：中央高校基本科研业务费专项资金(2019XZZX006-01);浙江省农业新品种选育重大科技专项重点研发计划项目(2016C02054-15)。

摘　　要：本研究采用直接测序法检测了无量山乌骨鸡磷脂酰肌醇-4-磷酸3-激酶催化亚基2型α(PIK3C2A)基因内含子的单核苷酸多态性(SNPs),并分析其与体尺和屠体性状的关联性。研究发现,在鸡PIK3C2A基因内含子上共检测到5个SNPs。关联分析表明,g.38511T>A位点与龙骨长和胫围相关(P<0.05),g.38573C>T位点与盆骨宽和胫围相关(P<0.05),g.38632T>C和g.38662A>G位点与胸角和胫围相关(P<0.05),g.38729C>T位点与胸角和盆骨宽相关(P<0.05或P<0.01);g.38511T>A位点与屠体率相关(P<0.05),g.38632T>C位点与活重、屠体重、半净膛重、全净膛重、腹脂重和腹脂率相关(P<0.05或P<0.01),g.38662A>G和g.38729C>T位点与活重、屠体重、半净膛重、全净膛重和胸肌率相关(P<0.05或P<0.01)。本研究结果表明,PIK3C2A基因的5个SNPs位点与无量山乌骨鸡的体尺和屠体性状相关(P<0.01),可作为鸡体尺和屠体性状的分子标记辅助选种。In this study,single nucleotide polymorphisms(SNPs)of phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha(PIK3C2A)gene were detected by direct sequencing in Wuliang Mountain Black-bone chickens,and its relationship with the body size and carcass traits was analyzed.Five SNPs were detected in intron region of the PIK3C2A gene.The correlation analysis showed that the g.38511T>A locus was associated with keel length and fossil bone length(P<0.05),g.38573C>T site associated with pelvic width and shank circumference(P<0.05),g.38632T>C and g.38662A>G sites associated with the breast angel and shank circumferences(P<0.05),and g.38729C>T site associated with breast angle and pelvic width(P<0.05 or P<0.01).As for the carcass traits,the g.38511T>A site was correlated with the percentage of half-eviscerated yield(P<0.05),g.38632T>C correlated with live weight,dressed weight,half-eviscerated weight,eviscerated weight,abdominal fat weight and the percentage of abdominal fat(P<0.05 or P<0.01),g.38662A>G and g.38729C>T sites associated with live weight,dressed weight,half-eviscerated weight,eviscerated weight and the percentage of breast muscle(P<0.05 or P<0.01).The above results indicated that the five SNPs of the PIK3C2A gene were correlated with the body size and carcass traits in chickens(P<0.01),and PIK3C2A gene could be the candidate gene for molecular marker-assisted selection in Wuliang Moutain black-bone chicken breeds.

关 键 词：PIK3C2A 多态性 体尺和屠体性状 无量山乌骨鸡 

分 类 号：S831.2[农业科学—畜牧学]