福建梅花山圈养华南虎幼虎与成年虎肠道菌群结构的比较研究  

Comparative Study of Intestinal Microbial Community Diversity Between Captive South China Tiger(Panthera tigris amoyensis)Cubs and Adults at Meihuashan,Fujian,China

作　　者：孙艳发[1,2,3] 张敏 陈腾腾[4] 傅文源 许卫华 吴琼 李焰[1,2,3] 朱雨婷 张雪梅 刘灵宇[1] 陈东虹 林开雄 林炜明 SUN Yanfa;ZHANG Min;CHEN Tengteng;FU Wenyuan;XU Weihua;WU Qiong;LI Yan;ZHU Yuting;ZHANG Xuemei;LIU Lingyu;CHEN Donghong;LIN Kaixiong;LIN Weiming(College of Life Science,Longyan University,Longyan,364012,China;Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology,Longyan,364012,China;Key Laboratory of Preventive Veterinary Medicine and Biotechnology(Longyan University),Longyan,364012,China;Fujian Meihuashan South China Tiger Breeding Institute,Shanghang,364201,China)

机构地区：[1]龙岩学院生命科学学院,龙岩364012 [2]福建省家畜传染病防治与生物技术重点实验室,龙岩364012 [3]预防兽医学与生物技术福建省高校重点实验室,龙岩364012 [4]福建梅花山华南虎繁育研究所,上杭364201

出　　处：《野生动物学报》2020年第4期912-918,共7页CHINESE JOURNAL OF WILDLIFE

基　　金：福建省中央引导地方科技发展专项(2018L3011);福建省自然科学基金面上项目(2018J01460);福建省中青年教师教育科研项目(JAT160482);福建省高校青年自然基金重点项目(JZ160482)。

摘　　要：通过对华南虎3只幼虎和5只成年虎粪便微生物16S rRNA基因V4区域进行高通量测序,分析华南虎幼虎和成年虎肠道菌群组成和差异。结果表明,8份样品共检测到501个OTUs,包含17个门和176个属。华南虎幼虎肠道细菌物种Beta多样性显著高于成年虎(P<0.05)。幼虎肠道梭菌纲(Clostridia)、梭菌目(Clostridiales)、梭菌科(Clostridiales)、梭菌属(Clostridiales)、消化链球菌科(Peptostreptococcaceae)以及Lachnoclostridium属细菌丰度显著低于成年虎(P<0.05)。华南虎幼虎与成年虎肠道细菌组成上在纲、目、科、属水平上存在差异显著物种(LDA score>4)。幼虎与成年虎肠道菌群组成的多样性与差异,可为不同年龄阶段华南虎的食物选择提供理论依据。Differences in intestinal microbial community diversity between South China tiger(Panthera tigris amoyensis)cubs and adults were analyzed based on high-throughput sequencing of the 16S rRNA gene V4 region of fecal microorganisms between 3 south cubs and 5 adults.Results showed that 501 OTUs were obtained from eight fecal samples,representing 17 floral phyla and 176 genera.The species beta diversity of intestinal samples of cub tigers was significantly higher than that of adult tigers(P<0.05).Abundances of Clostridia class,Clostridiales order,Clostridiales family,Clostridiales genus,Peptostreptococcaceae family,and Lachnoclostridium genus in tiger cubs was significantly lower than that of adult tigers(P<0.05).The intestinal floras of cub and adult tigers were different at class,order,family and genus levels(LDA score>4).Diversity of the intestinal microbial communities between cub and adult tigers can provide a theoretical basis for the selection of food for South China tigers of different ages.

关 键 词：华南虎 肠道菌群 16S rRNA 高通量测序 

分 类 号：Q93[生物学—微生物学]