miR-424和Lp-PLA2在子痫前期患者血清中的表达及妊娠结局的关系  

The Expressions of MiR-424 and Lp-PLA2 in Serum of Preeclampsia Patients and Their Relationships with Pregnancy Outcome

作　　者：曾诗晨 余得水[2] 曾丽[1] 严梅[1] 陈莉[1] 何英 ZENG Shichen;YU Deshui;ZENG Li;YAN Mei;CHEN Li;HE Ying(Obstetrics Department,the Second People′s Hospital,Yibin 644000,China;Department of Anesthesiology,the Second People′s Hospital,Yibin 644000,China)

机构地区：[1]宜宾市第二人民医院产科,四川宜宾644000 [2]宜宾市第二人民医院麻醉科,四川宜宾644000

出　　处：《标记免疫分析与临床》2020年第9期1542-1545,1623,共5页Labeled Immunoassays and Clinical Medicine

基　　金：四川省卫生和计划生育委员会科研项目(编号:150250)。

摘　　要：目的检测子痫前期孕妇血清微小RNA-424(miR-424)和脂蛋白相关磷脂酶A2(Lp-PLA2)的表达情况,并探讨二者在子痫前期发病过程中的作用及与孕妇妊娠结局的关系。方法选取2017年4月至2019年5月在本院妇产科确诊并住院分娩的71例子痫前期孕妇为研究对象,分为子痫前期重度组32例,轻度组39例,同期选取健康孕妇45例为对照组。采用实时荧光定量PCR法(qRT-PCR)检测各组血清miR-424表达水平;采用酶联免疫吸附(ELISA)法检测血清Lp-PLA2表达水平;并对子痫前期孕妇发生不良妊娠结局的相关影响因素进行Logistic回归分析。结果子痫前期重度组和轻度组血清miR-424表达水平显著低于对照组(P<0.05),血清Lp-PLA2表达水平显著高于对照组(P<0.05),重度组血清miR-424表达水平显著低于轻度组(P<0.05),血清Lp-PLA2表达水平显著高于轻度组(P<0.05);对照组、子痫前期轻度组及重度组早产、新生儿窒息、低体重儿以及巨大儿的发生率均依次升高,总不良结局数依次上升,且子痫前期重度组的总不良结局发生率明显高于对照组、轻度组(P<0.05);Logistic分析显示,血清miR-424表达水平是子痫前期孕妇发生不良妊娠结局的保护因素,血清Lp-PLA2是影响子痫前期孕妇发生不良妊娠结局的独立危险因素。结论miR-424在子痫前期患者血清中的表达水平明显降低,Lp-PLA2表达明显升高,且与子痫前期孕妇的妊娠结局密切相关,具有一定的诊断价值,提示血清miR-424和Lp-PLA2可作为子痫前期的病情评估及妊娠结局的潜在生物学指标。Objective To detect the expressions of microRNA-424(miR-424)and Lp-PLA2 in serum of preeclampsia pregnant women,and to explore their function roles in the pathogenesis of preeclampsia and relationships with pregnancy outcome.Methods From April,2017 to May,2019,71 cases of preeclampsia pregnant women who were confirmed and delivered in our hospital were selected as the study objects,and these patients were divided into the severe preeclampsia group with 32 cases and the mild group with 39 cases,while 45 cases of healthy pregnant women were enrolled as the control group.The expression of miR-424 was detected by real-time fluorescence quantitative PCR(qRT-PCR)and the expression of Lp-PLA2 in serum was detected by enzyme-linked immunosorbent assay(ELISA).Logistic regression analysis was used to analyze the related factors of adverse pregnancy outcome in preeclampsia pregnant women.Results The expression levels of miR-424 in the severe group and mild group were significantly lower than that in the control group(P<0.05),and the expression level of Lp-PLA2 in the severe group was significantly higher than that in the control group(P<0.05),while the expression level of miR-424 in the severe group was significantly lower than that in the mild group(P<0.05),and the expression level of Lp-PLA2 in the severe group was significantly higher than that in the mild group(P<0.05).The incidence rates of preterm delivery,neonatal asphyxia,low birth weight and macrosomia in the control group,mild and severe preeclampsia group increased in turn,so did the total number of adverse outcomes,and the total incidence of adverse outcomes in the severe preeclampsia group was significantly higher than those in the control group and mild group(P<0.05).Logistic analysis showed that the expression level of miR-424 in serum was the protective factor of adverse pregnancy outcome in preeclampsia pregnant women,and Lp-PLA2 was the independent risk factor of adverse pregnancy outcome in preeclampsia pregnant women.Conclusion The expression of miR-424

关 键 词：子痫前期 微小RNA-424 脂蛋白相关磷脂酶A2 表达 妊娠结局 

分 类 号：R714.244[医药卫生—妇产科学]