原发性干燥综合征患者唇腺组织中白细胞介素7的表达及临床意义  

Expression and clinical significance of interleukin⁃7 in the salivary glands of patients with primary Sjögren′s syndrome

作　　者：林玉娇[1] 王育凯 王友山[1] 谢奕文[1] Lin Yujiao;Wang Yukai;Wang Youshan;Xie Yiwen(Department of Stomatology,Shantou Central Hospital,Shantou 515031,China;Department of Rheumatology and Immunology,Shantou Central Hospital,Shantou 515031,China)

机构地区：[1]中山大学附属汕头医院·汕头市中心医院口腔科,汕头515031 [2]中山大学附属汕头医院·汕头市中心医院风湿免疫科,汕头515031

出　　处：《中华口腔医学研究杂志（电子版）》2020年第5期288-293,共6页Chinese Journal of Stomatological Research(Electronic Edition)

基　　金：广东省医学科研基金(A2019244);汕头市医疗科技计划(汕府科〔2015〕123号07、汕府科〔2019〕106号21)。

摘　　要：目的探讨原发性干燥综合征(pSS)患者唇腺组织中IL⁃7的表达及其与临床血清学参数间的关联。方法收集2015年1月至2017年12月汕头市中心医院pSS患者135例,以及同期唇外伤正常对照个体12例。免疫组织化学法检测两组个体唇腺组织中IL⁃7的表达,分别评估腺上皮细胞与浸润淋巴细胞IL⁃7的表达情况。Pearson相关分析唇腺组织IL⁃7的表达与抗核抗体(ANA)、C3、C4、C反应蛋白(CRP)、IgG等血清学指标间的关联性。结果IL⁃7在唇腺组织内淋巴细胞、腺泡导管及上皮细胞均可表达。浸润的淋巴细胞IL⁃7表达阳性率在pSS患者(88.14%,119/135)显著高于正常对照个体(8.3%,1/12),差异有统计学意义(χ2=46.82,P<0.001)。pSS患者唇腺组织中腺泡与导管上皮细胞与浸润淋巴细胞IL⁃7的表达相关(r=0.27,P=0.0012),未发现有浸润淋巴细胞IL⁃7表达与血清指标间的相关性。结论pSS患者唇腺组织中腺体上皮细胞、淋巴细胞IL⁃7异常高表达,且两者表达情况紧密相关,提示IL⁃7的表达可能参与了pSS腺体与淋巴细胞的相互作用,可作为pSS唇腺干预一个新的治疗靶点。Objective To investigate the expression of interleukin⁃7(IL⁃7)in the labial glands and its correlation with the clinical serum biomarkers in the patients with primary Sjögren′s syndrome.Methods A total of 135 patients with pSS from the Department of Rheumatology and Immunology of Shantou Central Hospital from January 2015 to December 2017 were recruited,while 12 normal individuals in the same period were included as control.Immunohistochemical method was used to detect the expression level of IL⁃7 in the glandular epithelial cells and infiltrated lymphocytes.The relationships between IL⁃7 and serum ANA,C3,C4,CRP and IgG were investigated.Results IL⁃7 was highly expressed in the epithelial cells and lymphocytes infiltrated in the pSS salivary glands.The positive expression of IL⁃7 in the pSS patients(88.14%,119/135)was significantly higher than that in the control(8.3%,1/12;χ2=46.82,P<0.001).The expression of IL⁃7 in the infiltrated lymphocytes was positively correlated with that in the epithelial cells(r=0.27,P=0.0012),while no correlation with the serum biomarkers,including ANA,C3,C4,CRP and IgG was found.Conclusions The increased expression of IL⁃7 in the glandular epithelial cells in patients with pSS was related to its expression in infiltrated lymphocytes,indicating a crosstalk between epithelial cells and lymphocytes in the immunopathology of pSS.IL⁃7 can be a therapeutic target for pSS.

关 键 词：干燥综合征 白细胞介素7 唇腺 

分 类 号：R593.2[医药卫生—内科学]