Extracellular vesicles based electrochemical biosensors for detection of cancer cells:A review  

作　　者：Hui Xie Kaili Di Rongrong Huang Adeel Khan Yanyan Xia Hongpan Xu Chang Liu Tingting Tan Xinyu Tian Han Shen Nongyue He Zhiyang Li 

机构地区：[1]Department of Clinical Laboratory,Nanjing Drum Tower Hospital Clinical College of Xuzhou Medical University,Nanjing 210008,China [2]Department of Clinical Laboratory,Yizheng Hospital of Nanjing Drum Tower Hospital Group.Yizheng 211900,China [3]Department of Clinical Laboratory,the Affiliated Drum Tower Hospital of Nanjing University Medical School,Nanjing 210008,China [4]State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,National Demonstration Center for Experimental Biomedical Engineering Education(Southeast University),Southeast University,Nanjing 210096,China [5]Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province,Hunan Key Laboratory of Biomedical Nanomaterials and Devices,Hunan University of Technology,Zhuzhou 412007,China

出　　处：《Chinese Chemical Letters》2020年第7期1737-1745,共9页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.61971216 and 61527806);National Key Research and Development Program of China(No.2017YFA0205301);the Jiangsu Province Medical Talent(No.ZDRCA2016065);the Key Research and Development Project of Jiangsu Province(No.BE2019603);the Health Care for Cadres Research Fund ofjiangsu Province(No.BJ16003);High-level Health Talent Project of Jiangsu Province(No.LGY2019001)。

摘　　要：Extracellular vesicles(EVs)derived from cancer cells are considered as ideal biomarker for liquid biopsy in cancer diagnosis,and are stable and abundant.Electrochemical methods for the detection of EVs are prefe rred over co nventional methods such as Western blotting and enzyme-linked immunosorbent assay for their high sensitivity and real-time detection.This article summaries studies proposing the electrochemical methods utilizing immunological and molecular methodologies for detecting EVs derived biomacromolecules such as miRNAs and transme mbrane protein for cancer diagnosis.Moreover,the electrochemical detection methods are compared and future prospects for the development of electrochemical methods for EVs detection are concluded.

关 键 词：Cancerous Extracellular vesicles ELECTROCHEMICAL Biosensors DETECTION 

分 类 号：O657.1[理学—分析化学] TP212[理学—化学] R730.4[自动化与计算机技术—检测技术与自动化装置]