鳗弧菌和副溶血弧菌对短蛸感染的病理学研究  

Pathological study on infection of Vibrio anguillarum and Vibrio parahemolyticus to Octopus ocellatus

作　　者：王金龙 冯艳微 李赞 王卫军 孙国华 徐晓辉 杨建敏 WANG Jin-long;FENG Yan-wei;LI Zan;WANG Wei-jun;SUN Guo-hua;XU Xiao-hui;YANG Jian-min(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Agricultural College,Ludong University,Yantai 264025,Shandong,China;Shanghai Aquaculture Engineering Technology Research Center,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]鲁东大学农学院,山东烟台264025 [3]上海海洋大学上海水产养殖工程技术研究中心,上海201306

出　　处：《山东大学学报（理学版）》2020年第9期102-110,共9页Journal of Shandong University(Natural Science)

基　　金：国家贝类产业技术体系基金资助项目(CARS-49);山东省自然科学基金资助项目(ZR2019BC052)。

摘　　要：为了今后养殖病害的防治,了解病原微生物在养殖过程中对头足类动物产生的影响,选择在海水养殖中发病频率较高的弧菌,包括鳗弧菌(Vibrio anguillarum)和副溶血弧菌(Vibrio Parahemolyticus),研究它们对短蛸的毒力水平。通过将稀释菌液直接注射短蛸腕部的方式,观察感染后短蛸的致死率、半致死时间,以及各菌对短蛸肝脏、鳃、鳃心、白体等器官结构的影响。结果显示:鳗弧菌和副溶血弧菌均对短蛸有较强的致病性,分别在60、48 h时达到半致死,且发病的短蛸活性减退,虚弱无力,腹部肿大,解剖可见部分内脏肿胀明显,如肝脏;通过对病变组织和健康组织的石蜡切片和HE染色,以及超显微结构的观察,发现患病短蛸的肝脏、鳃、鳃心和白体均出现不同程度组织、细胞结构上的破坏。以上结果表明这两种弧菌对短蛸均具有明显的致病性,副溶血弧菌更强一些,合适条件下将会成为短蛸规模化养殖的重要威胁之一。In order to control aquaculture diseases and understand the influence of pathogen microorganisms on cephalopoda culture,we selected vibrio with high incidence in seawater culture,including V.anguillarum and V.parahemolyticus,to study their virulence level and pathogenic mechanism against shortarm octopus.Through direct injection of diluted bacteria solution into shortarm octopus carpus arm,the death rate and half lethal time of shortarm octopus after infection were observed,as well as the effects of various bacteria on the structures of shortarm octopus liver,gill,gill heart and white body.The results showed that V.anguillarum and V.parahemolyticus were both highly pathogenic to shortarm octopus,reaching a semi-fatality rate at 60 h and 48 h,respectively.In addition,the activity of octopus was decreased,weakness and abdominal swelling were observed;Through the observation of paraffin sections and HE staining and ultramicroscopic structure of diseased and healthy tissues,it was found that the liver,gill,gill heart and white body of the diseased octopus showed different degrees of tissue or cell structure damage.The above results indicated that both V.anguillarum and V.parahemolyticus were significantly pathogenic to shortarm octopus,and the pathogenicity of V.parahaemolyticus might be stronger,which would be one of the greatest threats of shortarm octopus large-scale farming if the conditions were suitable.

关 键 词：短蛸 鳗弧菌 副溶血弧菌 感染 

分 类 号：Q17[生物学—水生生物学]