植物精油对鸽源白色念珠菌的体外抑菌作用及肉鸽攻毒保护试验  

Antibacterial Effect of Plant Essential Oils on Candida albicans in Vitro and Its Challenge Protection Test on Meat Pigeons

作　　者：郭罕星 王占新 鲁俊鹏 覃健萍 祁姣姣 胡学生 元波 陈彤 房向杰 胡文锋[1] GUO Hanxing;WANG Zhanxin;LU Junpeng;QIN Jianping;QI Jiaojiao;HU Xuesheng;YUAN Bo;CHEN Tong;FANG Xiangjie;HU Wenfeng(College of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642;Guangdong Provincial Key Laboratory of Livestock and Poultry Health Breeding and Environmental Control Enterprise,Wen's Food Group Co.,Ltd.,Yunfu,Guangdong 527439;Shenzhen Bioforte Biotechnology Co.,Ltd.,Shenzhen,Guangdong 518000)

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]温氏食品集团股份有限公司,广东省畜禽健康养殖与环境控制企业重点实验室,广东云浮527439 [3]生物源生物技术(深圳)股份有限公司,广东深圳518000

出　　处：《中国家禽》2020年第9期47-53,共7页China Poultry

基　　金：温氏食品集团股份有限公司科研项目(YQJS00164);生物源生物技术(深圳)股份有限公司开发项目(2019001)。

摘　　要：通过研究茶树精油、丁香精油、百里香油、柑橘油、小茴香油和艾叶精油对白色念珠菌的体外抑制作用,筛选出抑菌效果显著的植物精油组方。采用牛津杯法检测上述植物精油对白色念珠菌(ATCC10231和临床分离株)的抑菌效果;微量肉汤稀释法检测植物精油对白色念珠菌的最小抑菌浓度(MIC);观察植物精油组合处理鸽源白色念珠菌对细胞形态的影响;评价植物精油组合防治鸽子白色念珠菌感染的效果。结果显示:柑橘油对白色念珠菌的抑菌效果显著,对白色念珠菌ATCC10231及鸽源A、B和C型白色念珠菌的MIC分别为0.625、0.625、0.625和1.25μL/mL(v/v)。0.8%柑橘油和百里香油组合处理白色念珠菌可致菌丝破裂,胞内物质流出,假菌丝断裂。将0.8%柑橘油和百里香油组合饲喂攻毒患病的肉鸽,解剖嗉囊没有发现白色念珠菌病典型症状。结果表明,0.8%柑橘油和百里香油组合具有效防治肉鸽白色念珠菌病的应用潜力。To study the antibacterial effect of plant essential oils, which were extracted from tea tree, clove,thyme, citrus, fennel and wormwood leaves on Candida albicans in vitro, plant essential oil formula with significant antibacterial effect was selected. The Oxford cup method and broth microdilution assay were conducted to detect the inhibition zone and minimum inhibitory concentration(MIC) for the plant essential oils against C. albicans strains. The effect of plant essential oil combinations on morphology of Candida albicans and its prevention and control effect against Candida albicans from pigeons were observed. The results showed that citrus oil could significantly inhibit C. albicans growth with MICs against ATCC10231 and A, B, C genotypes of 0.625, 0.625, 0.625 and 1.25 μL/mL, respectively.Treatment of C. albicans with 0.8% citrus oil and thyme oil resulted in hyphal rupture, outflow of intracellular substances and pseudohyphae fracture. In addition, 0.8% citrus oil and thyme oil could protect pigeons against C. albicans infection.In conclusion, 0.8% citrus oil and thyme oil was a potential alternative to antifungals drugs which were used for prevention and control of C. albicans infection in pigeons.

关 键 词：植物精油 白色念珠菌 抑菌圈 肉鸽 

分 类 号：S859[农业科学—临床兽医学]