决明子水提物干预N-硝基-L-精氨酸甲酯诱导高血压模型大鼠血压的变化  

Effect of cassia seed aqueous extract on blood pressure level in N-nitro-L-arginine-methyl ester induced hypertensive rats

作　　者：叶泉英[1] 陈启生 李艳文 王婷 陈小艳[4] 岳赟[4] Ye Quanying;Chen Qisheng;Li Yanwen;Wang Ting;Chen Xiaoyan;Yue Yun(Medical Basic Experimental Teaching Center,Foshan University,Foshan 528000,Guangdong Province,China;Department of Hepatobiliary Surgery,Nanhai District People’s Hospital,Foshan 528000,Guangdong Province,China;College of Life Science and Biopharmaceuticals,Guangdong Pharmaceutical University,Foshan 510006,Guangdong Province,China;School of Stomatology,Foshan University,Foshan 528000,Guangdong Province,China)

机构地区：[1]佛山科学技术学院口腔医学院医学基础实验教学中心,广东省佛山市528000 [2]佛山市南海区人民医院肝胆外科,广东省佛山市528000 [3]广东药科大学生命科学与生物制药学院,广东省广州市510006 [4]佛山科学技术学院口腔医学院,广东省佛山市528000

出　　处：《中国组织工程研究》2021年第11期1705-1711,共7页Chinese Journal of Tissue Engineering Research

基　　金：佛山科学技术学院学术基金(gg08503),项目负责人:李艳文;2019大学生创新创业训练计划项目(XJ2018190),项目负责人:王婷。

摘　　要：背景:高血压现代诊疗理念是通过降压治疗保护靶器官、改善临床症状与最大程度减少临床事件。决明子在动物实验和临床实践中有降压作用,但是其是否有助于改善高血压血管功能障碍、氧化应激和终末器官损害仍有待于进一步研究。目的:评估决明子水提物对N-硝基-L-精氨酸甲酯诱导的高血压大鼠的降压功效。方法:灌胃N-硝基-L-精氨酸甲酯构建高血压大鼠模型,然后给予决明子水提物[500 mg/(kg·d)]治疗,均连续灌胃4周,1次/d。每周测量1次血压、心率。最后一次灌胃给药24 h后,检测血清谷草转氨酶、谷丙转氨酶、尿素、血肌酐、脂质谱以及肝脏和肾脏中丙二醛、一氧化氮、还原型谷胱甘肽水平、血管紧张素转化酶活性。通过免疫组化和实时定量PCR分析大鼠肾脏组织中内皮型一氧化氮合酶、诱导型一氧化氮合酶蛋白及基因表达。结果与结论:与高血压模型组相比,决明子水提物治疗4周有效降低了高血压大鼠的平均动脉压、舒张压、收缩压(P<0.05),并改善肝脏和肾脏标志物、脂质分布和氧化状态。此外,决明子水提物显著降低了高血压大鼠肝脏和肾脏中血管紧张素转化酶活性(P<0.05)。在决明子水提物处理的大鼠肾脏中,内皮型一氧化氮合酶的基因和蛋白表达均显著高于高血压模型组。以上结果表明,决明子水提物显示出相当大的降压潜力,其降压机制包括上调内皮型一氧化氮合酶表达、抗氧化和血管紧张素转化酶抑制作用。BACKGROUND:The concept of modern diagnosis and treatment of hypertension is to protect target organs,improve clinical symptoms and minimize clinical events through antihypertensive treatment.Cassia seed has an antihypertensive effect that has been confirmed in animal experiment and clinical practice,but whether it can improve the vascular dysfunction,oxidative stress and end organ damage of hypertension remains to be further studied.OBJECTIVE:To evaluate the antihypertensive effect of cassia seed water extract on hypertensive rats induced by N-nitro-L-arginine methyl ester.METHODS:A rat model of hypertension was established by intragastric administration of N-nitro-L-arginine methyl ester to observe the changes of blood pressure and heart rate after the intervention of cassia seed aqueous extract(500 mg/kg per day),once a day,for 4 continuous weeks.Blood pressure and heart rate were measured once a week.At 24 hours after the final administration,serum levels of aspartate aminotransferase,alanine aminotransferase,urea and serum creatinine,and lipid mass spectrometry were detected by an automatic biochemical analyzer.Malondialdehyde,nitric oxide,reduced glutathione levels and angiotensin converting enzyme activity in rat liver and kidney were determined by corresponding assay kits.The expression levels of endothelial nitric oxide synthase and inducible nitric oxide synthase protein and gene in rat kidney tissues were examined by immunohistochemistry as well as real-time quantitative PCR.RESULTS AND CONCLUSION:Compared with the model group,cassia seed aqueous extract treatment significantly reduced mean arterial pressure,diastolic pressure and systolic pressure(P<0.05)in hypertensive rats,and improved liver and renal markers,lipid distribution and oxidative status.In addition,cassia seed aqueous extract significantly reduced the activity of angiotensin-converting enzyme in the liver and kidney of hypertensive rats(P<0.05).The protein and mRNA expression levels of endothelial nitric oxide synthase in the kidney of ra

关 键 词：高血压 N-硝基-L-精氨酸甲酯 决明子 水提物 肾脏 肝脏 酶 大鼠 实验 

分 类 号：R452[医药卫生—治疗学] R363[医药卫生—临床医学]