NaCl胁迫下小麦根部耐盐性状QTL分析  

QTLs Analysis of Wheat Root Traits under NaCl Stress

作　　者：时晓磊 严勇亮[3] 石书兵[1] 张金波[3] 王继庆 谢磊[1,2] 耿洪伟 SHI Xiao-lei;YAN Yong-liang;SHI Shu-bing;ZHANG Jin-bo;WANG Ji-qing;XIE Lei;GENG Hong-wei(College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;Key Laboratory of Biotechnology,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Crop Germplasm Resources,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆农业大学生物技术重点实验室,乌鲁木齐830052 [3]新疆农业科学院农作物品种资源研究所,乌鲁木齐830091

出　　处：《新疆农业大学学报》2020年第1期1-9,共9页Journal of Xinjiang Agricultural University

基　　金：新疆维吾尔自治区高校科研计划自然科学重点项目(XJEDU2020I010);国家自然科学基金项目(3166100491)。

摘　　要：为了解小麦耐盐相关性状的遗传机理,定位盐胁迫下调控小麦根部性状的QTL(quantitative trait locus)位点,本研究利用浓度200 mmol/L NaCl溶液和正常营养液对Berkut×Worrakatta重组自交系群体(310份家系)的最长根长、根干重、根鲜重、根平均直径、根尖数、根表面积、根体积和总根长8个根部耐盐相关性状进行耐盐性测定,通过正常和盐胁迫条件下各根部性状和耐盐指数结合小麦50 K基因芯片对上述各性状进行QTL分析。结果表明,小麦根部性状表现出广泛的表型变异,变异系数16.3%~70.2%。检测到10个分别与总根长、根表面积、根体积、根尖数、根鲜重和最长根长等6个小麦根部性状相关的QTL,分布于小麦1A、2A、6A、3B、4B、1D、2D和5D染色体,单个QTL可解释3.69%~4.95%表型变异,其中总根长、最长根长、根尖数分别有3、2、2个不同QTL位点;在6A染色体51 cM处同时检测到与总根长和根表面积相关的QTL位点QTRL.xjau-6 AS/QSA.xjau-6 AS,位于AX-179476814和AX-179558749标记间,贡献率为3.77%~4.66%;检测到QRDW.xjau-3BL和QRTN.xjau-2AS 2个小麦根部性状耐盐指数的QTL,分别位于2A染色体首端和3B染色体116 cM处,单个QTL可解释4.18%~4.83%的表型变异。对这些QTL位点进行基因发掘,筛选到10个可能与小麦根部耐盐性状有关的候选基因。TraesCS2A01G024100可以作为离子阻断剂,TraesCS1A01G307800和TraesCS6A01G092700参与调控植物包括抗逆反应在内的许多生命进程,这些候选基因可作为耐盐性重要基因。In order to understand the genetic mechanism of wheat salt-tolerance-related traits and locate quantitative trait locus(QTLs)that regulate wheat root traits under salt stress,this study used a 200 mmol/L NaCl solution and normal nutrient solution to reconstruct the Berkut×Worrakatta inbred line population(310 lines)of longest root length,root dry weight,fresh root weight,average root diameter,number of root tips,root surface area,root volume,and total root length of eight root-related traits for measurement of salt tolerance.The root traits under normal and salt stress conditions and the salt tolerance index of each root trait were combined with wheat 50 K chip to perform QTL analysis on the above eight root salt tolerance traits.The results of the study indicated that the wheat root traits exhibited a wide range of phenotypic variations,with coefficients of variation ranging from 16.3%to 70.2%.Ten QTLs were detected for 6 wheat root traits including total root length,root surface area,root volume,root tip number,root fresh weight,and longest root length.These QTLs were distributed in wheat 1A,2A,6A,3B,4B,1D,2D and 5D chromosomes,a single QTL could explain 3.69%-4.95%of the phenotypic variation,in which the total root length,the longest root length,and the number of root tips have 3,2,2 different QTL sites;The QTLs QTRL.xjau-6AS and QSA.xjau-6AS with total root length and root surface area were simultaneously detected at 51 cM on chromosome 6A,located between the AX-179476814-AX-179558749 markers,with a contribution rate of 3.77%-4.66%;QRDW.xjau-3BL and QRTN.xjau-2AS 2 QTLs for the salt tolerance index of wheat root traits were detected,located at the beginning of chromosome 2A and 116 cM of chromosome 3B respectively.A single QTL could account for 4.18%-4.83%phenotypic variation.Gene discovery of these QTL loci led to the selection of 10 candidate genes that might be related to the salt tolerance of wheat roots.TraesCS2A01G024100 could be used as an ion blocker.TraesCS1A01G307800 and TraesCS6A01G092700 were inv

关 键 词：小麦 耐盐性 根部性状 QTL 候选基因 

分 类 号：S512.103.2[农业科学—作物学]