电针结合被动牵伸运动对废用性肌萎缩小鼠骨骼肌恢复的影响  

Effects of electroacupuncture combined with passive stretch exercise on disused muscle atrophy and expression of skeletal troponin in mice

作　　者：白震民 王哲培 郭明航 张灵婕 徐继来 于亮[3] 李春日[4] BAI Zhen-min;WANG Zhe-pei;GUO Ming-hang;ZHANG Ling-Jie;XU Ji-lai;YU Liang;LI Chun-ri(College of Sport and Rehabilitation,Beijing Sport University,Beijing 100084.China;School&Hospital of Stomatology,Wuhan University,Wuhan 420079;Sport Science College,Beijing Sport University,Beijing 100084;College of Acupuncture-moxibustion and Massage,Liaoning University of Traditional Chinese Medicine,Shenyang 110847)

机构地区：[1]北京体育大学运动医学与康复学院,北京100084 [2]武汉大学口腔医学院,武汉420079 [3]北京体育大学运动人体科学学院,北京100084 [4]辽宁中医药大学针灸推拿学院,沈阳110847

出　　处：《针刺研究》2020年第9期720-725,共6页Acupuncture Research

基　　金：中央高校基本科研业务费专项资金资助课题(No.2019PT104)。

摘　　要：目的:观察电针和被动牵伸运动对小鼠后肢石膏固定制动所致腓肠肌、比目鱼肌废用性肌萎缩的影响,探讨电针结合被动牵伸运动对废用性肌萎缩小鼠骨骼肌恢复的作用。方法:将50只C57BL/6小鼠随机分为空白对照组、模型对照组、电针干预组、被动运动组和结合干预组,每组10只。除空白对照组外,其余4组均采用石膏固定方法制备废用性骨骼肌萎缩模型。电针干预组给予双侧"足三里"电针治疗,每次10 min,1次/d;被动运动组双下肢进行被动牵伸运动,每次10 min,1次/d;结合干预组每次电针干预5 min,被动牵伸5 min,1次/d。各组均干预4周。用肌肉形态ATPase法观察各组小鼠肌纤维横截面积的变化,Western blot法检测慢速骨骼肌型肌钙蛋白(TNNI1)、快速骨骼肌型肌钙蛋白(TNNI2)的表达。结果:与空白对照组比较,模型对照组小鼠比目鱼肌与腓肠肌的快、慢肌纤维横截面积显著下降(P<0.05,P<0.01)。与模型对照组比较,电针干预组小鼠腓肠肌快、慢肌纤维横截面积显著升高(P<0.05);结合干预组小鼠比目鱼肌、腓肠肌的快、慢肌纤维横截面积及腓肠肌TNNI1、TNNI2蛋白相对表达量显著升高(P<0.05,P<0.01)。结论:电针结合被动牵伸运动的治疗较单纯被动牵伸运动,能显著促进肌肉横截面积的恢复,同时还可以促进腓肠肌肌钙蛋白的表达,提示临床采用电针结合被动牵伸运动可促进固定制动导致的肌萎缩康复。Objective To observe the efficacy of electroacupuncture(EA)plus passive stretch exercise in the treatment of disused atrophy of gastrocnemius and soleus muscles in mice.Methods Fifty C57 BL/6 mice were randomly and equally divided into 5 groups:blank control,model,passive stretch exercise(exercise),EA and EA+exercise groups.The muscular atrophy model was established by fixing the gastrocnemius and soleus muscles with plaster immobilization(by putting the right leg into a plastic vial and then twining the vial with medical plaster bandage from the ankle upwards to the thigh and groin to maintain the knee-joint flexion and ankle joint plantar flexion for 7 days).EA(2 Hz/100 Hz,1 mA)was applied to bilateral"Zusanli"(ST36)for 10 min,once a day for 4 weeks.For mice with the passive exercise,the plastic vial was removed first,followed by pulling out the hindleg to seize the toes to stretch them until the right hindleg is fully extended,then,pushed the leg towards the body.The procedures were repeated once again and again for 10 min.The exercise was conducted once daily,for 4 weeks.The cross-sectional area of fast and slow muscle fibers of the soleus and gastrocnemius was measured under electronic microscope after ATPase histochemical stain and the expression of slow skeletal muscle troponin(TNNI1)and fast skeletal muscle troponin(TNNI2)in the soleus and gastrocnemius was detected by Western blot.Results Compared with the blank control group,the cross-sectional areas of the fast and slow muscle fibers of the soleus and gastrocnemius muscles were significantly decreased in the model group(P<0.05,P<0.01).Following the interventions,the cross-sectional areas of the fast and slow muscle fibers of soleus muscle in the EA+exercise group,and those of the fast and slow muscle fibers of the gastrocnemius muscle in the EA and EA+exercise groups,and the expression levels of TNNI1 and TNNI2 proteins in the gastrocnemius muscle of the EA+exercise group were significantly increased in comparison with the model group(P<0.05,P<0.01).Co

关 键 词：电针 被动牵伸 废用性肌萎缩 比目鱼肌 腓肠肌 慢速骨骼肌型肌钙蛋白 快速骨骼肌型肌钙蛋白 

分 类 号：R245.97[医药卫生—针灸推拿学]