西南桦肉桂酰辅酶A还原酶基因的克隆与表达分析  

Cloning and Expression Analysis of Cinnamoyl-CoA reductase Gene of Betula alnoides

作　　者：李云琴 金智伟 严毅 原晓龙 王毅[1,3] 张劲峰 LI Yun-qin;JIN Zhi-wei;YAN Yi;YUAN Xiao-long;WANG Yi;ZHANG Jin-feng(Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China;Kunming Haikou Forest Farm,Kunming Yunnan 650114,P.R.China;The Key Laboratory of Forestry Plant Cultivation and Utilization,The Key Laboratory of Rare and Endangered Forest Plants of National Forestry and Grassland Administration,Yunnan Academy of Forestry and Grassland,Kunming Yunnan 650201,P.R.China)

机构地区：[1]云南省林业和草原科学院,云南昆明650201 [2]昆明市海口品林场,云南海品650114 [3]云南省森林植物培育与开发利用重点实验室,国家林草局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明650201

出　　处：《西部林业科学》2020年第5期18-22,29,共6页Journal of West China Forestry Science

基　　金：云南省重点研发计划-农业领域(2018BB006);云南省重点研发计划-农业领域(2018BB007);APFNet(2019P3-CAM);分子生物学研究团队(LKYTD-2020-001)。

摘　　要：为研究肉桂酰辅酶A还原酶基因(CCR)在西南桦木质素合成中的作用机制,采用RT-PCR技术从西南桦中克隆得到1个具完整开放阅读框的肉桂酰CoA还原酶基因(BaCCR),该基因全长975 bp,编码324个氨基酸。序列比对分析显示西南桦CCR蛋白序列与胡桃、欧洲栓皮栎、梅、甜樱桃的蛋白序列相似性均在80%以上。BaCCR拥有CCR蛋白的NADP结合域和底物结合域KNWYCYGK。进化分析表明,BaCCR与梅、甜樱桃、亚洲棉、葡萄聚为一个枝系。转录模式分析表明,BaCCR基因在根和小枝中高量表达。To study the mechanism of Cinnamoyl Cinnamoyl-CoA reductase Gene in lignin synthesis of Betula alnoides,the cinnamoyl Cinnamoyl-CoA reductase gene(BaCCR)was cloned first from B.alnoides using RT-PCR.The gene has a total length of 975 bp and encodes 324 amino acids.Sequence alignment analysis showed that the similarity of CCR protein sequence between B.alnoides and Juglans regia(XP_018844471.1)and Quercus suber(XP_023912329.1)was more than 80%.The amino acids contained a NAD(P)-binding domain and a substrate-binding domain typical to CCR.The phylogenetic analysis revealed that BaCCR and Prunus mume(XP_008231479.1),Prunus avium(XP_021834723.1),Gossypium arboreum(XP_017617353.1),Vitis vinifera(XP_010648113.1)clustered together.BaCCR had the highest expressed in roots and branchlets with high lignification.

关 键 词：西南桦 肉桂酰辅酶A还原酶 基因克隆 基因表达 

分 类 号：S796[农业科学—林木遗传育种] S718.46[农业科学—林学]