延胡索乙素对链脲佐菌素致糖尿病大鼠的影响  

Effects of tetrahydropalmatine on streptozotocin-induced diabetes rats

作　　者：宋亚刚 白明[1] 苗明三[1] SONG Ya-gang;BAI Ming;MIAO Ming-san(Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,郑州450046

出　　处：《中华中医药杂志》2020年第8期3837-3841,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重大新药创制项目(No.2017ZX09301071);国家国际合作基地(No.2016-151)。

摘　　要：目的:延胡索乙素对糖尿病(DM)大鼠降血糖作用及其机制进行初步研究。方法:采用链脲佐菌素(STZ)诱导建立DM大鼠模型;除空白组,造模大鼠根据血糖值随机分为模型组,盐酸二甲双胍组(330mg/kg),延胡索乙素大、中、小剂量组(25.00、12.50、6.25mg/kg),每组10只,除空白组和模型组(0.1mL/10g)给予同体积0.9%氯化钠溶液外,其余各组每天灌胃相应药物,1次/d,连续31d。观察大鼠动态空腹血糖值、胰岛素和C肽、丙二醛(MDA)、超氧化物歧化酶(SOD)、白细胞介素-1β(IL-1β)、γ-干扰素(IFN-γ)水平,胰胰腺组织病理变化及Bax和Bcl-2的表达。结果:与模型组比较,延胡索乙素大、中剂量干预后血糖值降低,MDA、IL-1β和IFN-γ降低,血清胰岛素、C肽、SOD、胰腺指数升高;胰腺组织病变改善,Bax降低、Bcl-2/Bax升高。结论:延胡索乙素对STZ诱导DM大鼠模型具有一定的降糖作用,其作用机制可能与抑制大鼠体内炎症因子和增强机体抗氧化能力、抑制胰岛细胞凋亡有关。Objective:To investigate the hypoglycemic effect of tetrahydropalmatine on diabetic rats,and to study its mechanism.Methods:The rat model of diabetes mellitus induced by streptozotocin(STZ)was established.According to blood glucose level,except for the blank control group,model rats were randomly divided into model group,metformin hydrochloride group(330 mg/kg,equivalent to clinical dose 10 times),and tetrahydropalmatine high,medium and low dose group(25.00,12.50,6.25 mg/kg),10 mice in each group.Except for the blank and model group(0.1 mL/10 g)were given the same volume of normal saline,the other groups were given the corresponding drugs once a day,once a day for 31 days.The dynamic fasting blood glucose,insulin and C-peptide,MDA,SOD,IL-1β,INF-γlevels,pathological changes of pancreatic pancreas and the expression of Bax and Bcl-2 were observed.Results:Compared with the model group,After intervention with high and medium dose tetrahydropalmatine,the blood glucose level decreased,MDA,IL-1βand INF-γdecreased,serum insulin,C-peptide,SOD and pancreatic index increased;pancreatic tissue lesions improved,Bax decreased,Bax/Bcl-2 increased.Conclusion:Tetrahydropalmatine has a hypoglycemic effect on STZ-induced diabetic rat model,and its mechanism may be related to inhibiting inflammatory factors in rats and enhancing the body’s antioxidant capacity and inhibiting islet cell apoptosis.

关 键 词：延胡索乙素 链脲佐菌素 糖尿病 降糖作用 

分 类 号：R285.5[医药卫生—中药学]