慢性胃炎浊毒内蕴证与Th1/Th2平衡关系的研究  

Study on the relationship between turbid toxin accumulation syndrome and Th1/Th2 balance in chronic gastritis

作　　者：娄莹莹[1] 李佃贵[1] 郭喜军[1] 霍永利[1] 赵亚萍[1] 孙润雪 张晓艳[1] LOU Ying-ying;LI Dian-gui;GUO Xi-jun;HUO Yong-li;ZHAO Ya-ping;SUN Run-xue;ZHANG Xiao-yan(Department 3 of Spleen and Stomach Diseases,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050017,China)

机构地区：[1]河北省中医院脾胃病三科,石家庄050017

出　　处：《中华中医药杂志》2020年第8期4132-4134,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医临床研究基地建设项目(No.国中医药办科技函[2018]18);河北省科学技术厅项目(No.17277709D)。

摘　　要：目的:探讨慢性胃炎(CG)浊毒内蕴证与Th1/Th2平衡的关系。方法:收集CG浊毒内蕴证患者60例(包括毒重于浊9例,浊重于毒21例,浊毒并重30例),以10名健康志愿者为健康对照组。采用C14呼气试验判定幽门螺杆菌(Hp)感染率,用ELISA试剂盒检测血清Th1型细胞因子干扰素-γ(IFN-γ)、白细胞介素(IL)-12和Th2型细胞因子IL-4、IL-10的表达。结果:与健康对照组比较,CG浊毒内蕴证患者外周血IFN-γ表达显著升高(P<0.05);浊毒内蕴证患者Hp阳性和阴性组间比较无显著差异。浊毒内蕴证各亚型组之间IFN-γ、IL-4水平及IFN-γ/IL-4比值比较,浊毒并重组各指标水平较其它两组高,但差异均无统计学意义。与健康对照组比较,浊毒内蕴组患者Hp感染率明显增高,其中毒重于浊、浊重于毒,浊毒并重Hp感染率分别为33.3%(3/9)、42.8%(9/21)、46.7%(14/30),均较健康对照组显著升高(P<0.05)。结论:慢性胃炎浊毒内蕴证与Th1/Th2动态平衡密切相关,"浊毒"可能是介导Th1型细胞因子发生免疫反应的关键病理因素。Objective:To discuss the relationship between chronic turbid toxin accumulation syndrome and Th1/Th2 balance from a microcosmic point of view.Methods:Sixty cases of chronic gastritis with turbid toxin accumulation syndrome were collected,including 9 cases with predominant toxicity(TTS-TO),21 cases with predominant turbidity(TTS-TU)and 30 cases with simultaneous onset of toxicity and turbidity(TTS-TT).Besides 10 healthy volunteers were recruited as the healthy control group.Determination of Hp infection rate by C14 breath test.ELISA kit was used to detect the expression of interferon gamma(IFN-γ),interleukin(IL)-12 and Th2 cytokine IL-4,IL-10 in serum Th1 cells.Results:Compared with the healthy control group,the expression of IFN-γin peripheral blood of the patients with turbid toxin internal accumulation syndrome increased(P<0.05).There was no significant difference between Hp positive and negative groups.The levels of IFN-γ,IL-4 and the ratio of IFN-γ/IL-4 in turbid toxin were higher than those two groups,but there was no significant difference between the three groups.The infection rate of Hp in turbid toxin accumulation group was significantly higher than that in healthy control group(P<0.05).The Hp infection rate was 33.3%(3/9)in TTS-TO,42.8%(9/21)in TTS-TU,46.7%(14/30)in TTS-TT.Compared with the healthy control group,the difference was statistically significant(P<0.05).Conclusion:The accumulation of turbid toxin in chronic gastritis is closely related to the dynamic balance of Th1/Th2.Turbid toxin may be the key pathological factor mediating the immune response of Th1 cytokines.

关 键 词：慢性胃炎 浊毒内蕴证 TH1/TH2 

分 类 号：R259[医药卫生—中西医结合]