溴化呋喃酮协同解毒祛瘀滋阴方治疗MRL/lpr狼疮小鼠的研究  

Study on bromofuranone in combination with Jiedu Quyu Ziyin Formula in treating MRL/lpr mice

作　　者：何志兴[1] 王明珠 谢朵丽 温成平[1] 邵铁娟[1] HE Zhi-xing;WANG Ming-zhu;XIE Duo-li;WEN Cheng-ping;SHAO Tie-juan(College of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学基础医学院,杭州310053

出　　处：《中华中医药杂志》2020年第8期4139-4142,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81703864);浙江省自然科学基金项目(No.LQ17H270002);中国博士科学基金项目(No.2016M601979)。

摘　　要：目的:探究溴化呋喃酮协同解毒祛瘀滋阴方治疗MRL/lpr狼疮小鼠的影响,从肠道菌群角度分析可能的机制。方法:运用Illumina MiSeq高通量测序技术检测狼疮小鼠的肠道菌群结构,利用LEfSe分析挖掘狼疮模型小鼠与中药组、溴化呋喃酮组及联合用药组的狼疮小鼠的差异菌群。结果:联合用药缓解狼疮小鼠病情的效果优于单用解毒祛瘀滋阴方;溴化呋喃酮不能缓解小鼠狼疮病情,但能上调Lactobacillus丰度和下调Rikenella丰度,利于联合用药狼疮小鼠的病情缓解。结论:溴化呋喃酮协同解毒祛瘀滋阴方通过调节肠道菌群治疗狼疮。Objective:To explore the effect of bromofuranone in combination with Jiedu Quyu Ziyin Formula in the treatment of MRL/lpr mice and reveal the possible mechanism from the perspective of gut microbiome.Methods:Illumina MiSeq sequencing was used to explore the microbial communities of MRL/lpr mice.LEfSe analysis was used to reveal the significantly different microbial species between the model mice and Jiedu Quyu Ziyin Formula treated mice,bromofuranone treated mice,the combined drugs reated mice.Results:The efficacy of combined drugs in relieving lupus disease was better than that of Jiedu Quyu Ziyin Formula.Although bromofuranone could not alleviate lupus activity,it could increase the abundance of Lactobacillus and decrease the abundance of Rikenella,which was beneficial to low lupus activity in the combined drugs-treated mice.Conclusion:Bromofuranone in combination with Jiedu Quyu Ziyin Formula can promote treating lupus by regulating gut microbiota.

关 键 词：MRL/LPR小鼠 解毒祛瘀滋阴方 溴化呋喃酮 肠道菌群 

分 类 号：R593.241[医药卫生—内科学]