基于响应面法的马比木中喜树碱提取工艺的优化  

Optimization of camptothecin extraction process from Nothapodytes pittosporoides by Box-Behnken response surface method

作　　者：林劲草 肖莉[2,3] 吴酬飞 张绍勇[2,3] 杨惠宁 张立钦 LIN Jincao;XIAO Li;WU Choufei;ZHANG Shaoyong;YANG Huining;ZHANG Liqin(College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;College of Life Sciences,Huzhou University,Huzhou 313000,Zhejiang,China;Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province,Huzhou University,Huzhou 313000,Zhejiang,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州311300 [2]湖州师范学院生命科学学院,浙江湖州313000 [3]湖州师范学院,浙江省媒介生物学与病原控制重点实验室,浙江湖州313000

出　　处：《浙江农林大学学报》2020年第5期1014-1019,共6页Journal of Zhejiang A&F University

基　　金：浙江省重点研发计划项目(2019C02030,2019C02024);“十三五”国家重点研发计划子课题资助项目(2018YFD0600201-3)。

摘　　要：【目的】应用响应面法及超声波辅助优化马比木Nothapodytes pittosporoides中喜树碱的提取工艺。【方法】采用高效液相色谱法测定喜树碱含量,以市售马比木为研究对象,在单因素实验的基础上,选取甲醇体积分数、液料比及超声时间为影响因素,喜树碱提取率为响应值,通过Box-Behnken响应面法设计3因素3水平实验,确定最优提取工艺。【结果】最优提取工艺为甲醇体积分数46.4%,超声时间26.1 min,液料比21.20 mL·g^-1,喜树碱的提取率为0.0844%,二项式拟合相关系数R2为0.9693,预测值与实验值偏差为3.2%。【结论】采用Box-Behnken优化马比木中喜树碱提取工艺,精确度高,预测值与实验值吻合度较高,预测性良好。[Objective]This study aims to optimize extraction process of camptothecin from Nothapodytes pittosporoides by response surface method and ultrasonic wave.[Method]The content of camptothecin was determined by high performance liquid chromatography,and N.pittosporoides wood available on the market was used as the research object.Based on the single factor experiment,methanol concentration,liquid to solid ratio and ultrasonic time were taken as the influencing factors,and the extraction rate of camptothecin as the response value.Box-Behnken response surface method was used to design the experiment with 3 factors and 3 levels,so as to determine the optimal extraction process.[Result]The optimal extraction process was as follows:methanol concentration 46.4%,ultrasonic time 26.1 min,liquid to solid ratio 21.20 mL·g^-1,extraction rate of camptothecin 0.0844%,binomial fitting correlation coefficient R20.9693,and deviation between the predicted value and the experimental value 3.2%.[Conclusion]Box-Behnken can be used to optimize the extraction process of camptothecin from N.pittosporoides,with high precision and good predictability.

关 键 词：植物学 喜树碱 马比木 高效液相色谱 响应面法 

分 类 号：S718.43[农业科学—林学]