FTY720,a sphingosine analog,altered placentome histoarchitecture in ewes  

作　　者：Kathrin A.Dunlap Bryan G.White David W.Erikson M.Carey Satterfield Christiane Pfarrer Guoyao Wu Fuller W.Bazer Robert C.Burghardt Kayla J.Bayless Greg A.Johnson 

机构地区：[1]Department of Animal Science,Texas A&M University,College Station,TX 77843,USA [2]Okanagan College Salmon Arm Campus,Salmon Arm,British Columbia,Canada [3]Endocrine Technologies Core,Oregon National Primate Research Center,Oregon Health&Science University,Beaverton,OR,USA [4]Department of Anatomy,University of Veterinary Medicine Hannover,Bischofsholer Damm 15,30173 Hannover,Germany [5]Department of Veterinary Integrative Biosciences,College of Veterinary Medicine and Biomedical Sciences,Texas A&M University,College Station,TX 77843,USA [6]Department of Molecular and Cellular Medicine,Texas A&M Health Science Center,College Station,TX 77843,USA

出　　处：《Journal of Animal Science and Biotechnology》2020年第2期380-392,共13页畜牧与生物技术杂志（英文版）

基　　金：National Research Initiative Competitive Grant No.2009-35203-05725(KJB and GAJ);Fellowship No.2008-35203-18830(KAD)from the USDA National Institute of Food and Agriculture.

摘　　要：Background:The lysosphingolipid,sphingosine-1-phosphate,is a well-described and potent pro-angiogenic factor.Receptors,as well as the sphingosine phosphorylating enzyme sphingosine kinase 1,are expressed in the placentomes of sheep and the decidua of rodents;however,a function for this signaling pathway during pregnancy has not been established.The objective of this study was to investigate whether sphingosine-1-phosphate promoted angiogenesis within the placentomes of pregnant ewes.Ewes were given daily jugular injections of FTY720(2-amino-2[2-(−4-octylphenyl)ethyl]propate-1,3-diol hydrochloride),an S1P analog.Results:FTY720 infusion from days 30 to 60 of pregnancy did not alter maternal organ weights nor total number or mass of placentomes,but did alter placentome histoarchitecture.Interdigitation of caruncular crypts and cotyledonary villi was decreased,as was the relative area of cotyledonary tissue within placentomes.Also,the percentage of area occupied by cotyledonary villi per unit of placentome was increased,while the thickness of the caruncular capsule was decreased in ewes treated with FTY720.Further,FTY720 infusion decreased the number and density of blood vessels within caruncular tissue near the placentome capsule where the crypts emerge from the capsule.Finally,FTY720 infusion decreased asparagine and glutamine in amniotic fluid and methionine in allantoic fluid,and decreased the crown rump length of day 60 fetuses.Conclusions:While members of the sphingosine-1-phosphate signaling pathway have been characterized within the uteri and placentae of sheep and mice,the present study uses FTY720 to address the influence of S1P signaling on placental development.We present evidence that modulation of the S1P signaling pathway results in the alteration of caruncular vasculature,placentome architecture,abundance of amino acids in allantoic and amniotic fluids,and fetal growth during pregnancy in sheep.The marked morphological changes in placentome histoarchitecture,including alteration in the vasculature,m

关 键 词：Placentome Pregnancy SHEEP Sphingosine1 phosphate(S1P) 

分 类 号：S826[农业科学—畜牧学]