Early inoculation with caecal fermentation broth alters small intestine morphology,gene expression of tight junction proteins in the ileum,and the caecal metabolomic profiling of broilers  

作　　者：Yujie Gong Wenrui Xia Xueting Wen Wentao Lyu Yingping Xiao Hua Yang Xiaoting Zou 

机构地区：[1]Key Laboratory for Molecular Animal Nutrition of Ministry of Education,Feed Sciences Institute,College of Animal Sciences,Zhejiang University(Zijingang Campus),Hangzhou 310058,China [2]State Key Laboratory for Quality and Safety of Agro-products,Institute of Quality and Standards for Agro-products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China

出　　处：《Journal of Animal Science and Biotechnology》2020年第2期440-452,共13页畜牧与生物技术杂志（英文版）

基　　金：supported by the National Key R&D Program of China(2017YFD0500501);State Key Laboratory for Quality and Safety of Agroproducts(2010DS700124-ZZ1905).

摘　　要：Background:The establishment of stable microbiota in early life is beneficial to the individual.Changes in the intestinal environment during early life play a crucial role in modulating the gut microbiota.Therefore,early intervention to change the intestinal environment can be regarded as a new regulation strategy for the growth and health of poultry.However,the effects of intestinal environmental changes on host physiology and metabolism are rarely reported.This study was conducted to investigate the effects of early inoculation with caecal fermentation broth on small intestine morphology,gene expression of tight junction proteins in the ileum,and cecum microbial metabolism of broilers.Results:Our data showed that early inoculation with caecal fermentation broth could improve intestine morphology.The small intestine villus height was significantly increased(P<0.05)in the intervened broilers compared to the control group,especially on day 28.A similar result was observed in the ratio of villus height to crypt depth(P<0.05).Meanwhile,we found early inoculation significantly increased(P<0.05)the expression levels of zonula occludens-1(ZO1)on days 14 and 28,claudin-1(CLDN1)on day 28,whereas the gene expression of claudin-2(CLDN2)was significantly decreased(P<0.05)on days 14 and 28.Gas chromatography time-of-flight/mass spectrometry(GC-TOF/MS)technology was further implemented to systematically evaluate the microbial metabolite profiles.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)displayed a distinct trend towards separation between the fermentation broth group(F group)and the control group(C group).The differentially expressed metabolites were identified,and they were mainly functionally enriched in beta-alanine metabolism and biosynthesis of unsaturated fatty acids.In addition,1,3-diaminopropane was selected as a key biomarker that responded to early inoculation with caecal fermentation broth.Conclusions:These results provide insight into intestinal metabolomi

关 键 词：BROILERS Early inoculation Gene expression Intestine morphology Metabolomics profiling 

分 类 号：S831[农业科学—畜牧学]