转Cry1Ab-ma基因玉米CM8101对草地贪夜蛾1龄和2龄幼虫的抗性  

Resistance of transgenic maize CM8101 with Cry1Ab-ma gene against the 1st and 2nd instar larvae of fall armyworm Spodoptera frugiperda

作　　者：武奉慈[1] 翁建峰[2] 李新海[2] 尹俊琦[1] 宋新元[1] WU Fengci;WENG Jianfeng;LI Xinhai;YIN Junqi;SONG Xinyuan(Institute of Agricultural Biotechnology,Jilin Academy of Agricultural Sciences,Changchun 130033,Jilin Province,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《植物保护学报》2020年第4期815-821,共7页Journal of Plant Protection

基　　金：国家转基因生物新品种培育重大专项(2016ZX08011-003)。

摘　　要：为明确转Bt基因玉米对草地贪夜蛾Spodoptera frugiperda的抗性,采用室内玉米离体组织生测法测定取食转Cry1Ab-ma基因抗虫玉米CM8101心叶、花丝和籽粒后草地贪夜蛾1龄和2龄幼虫存活数量、存活幼虫的体重、蛹重、化蛹和羽化等参数,分析转Cry1Ab-ma基因抗虫玉米CM8101三种组织对草地贪夜蛾1龄和2龄幼虫的抗性。结果显示,转Cry1Ab-ma基因玉米CM8101心叶可完全杀死草地贪夜蛾1龄幼虫,2龄幼虫有存活,存活率为10.0%,但不能化蛹与羽化;花丝可杀死大部分草地贪夜蛾1龄幼虫,存活率为3.3%,并阻断其生活史,但对2龄幼虫杀伤力弱,存活率为73.3%,存活幼虫化蛹率和羽化率分别为20.0%和13.3%;籽粒可完全杀死草地贪夜蛾1龄幼虫,2龄幼虫有存活,存活率为13.3%,有少量可化蛹,化蛹率为3.3%、有少量羽化,羽化率为3.3%。表明转Cry1Ab-ma基因玉米CM8101在短期内对草地贪夜蛾1龄和2龄幼虫有良好的控制作用。To clarify the resistance effects of transgenic Bt maize against fall armyworm Spodoptera frugiperda, the vitro tissues bioassay was conducted in the laboratory. The 1 st and 2 nd instar larvae of S.frugiperda were feed with heart leaves, maize silks and seeds, the survival number of 1 st and 2 nd instar larvae, weight of surviving larvae, pupal weight, number of pupation and eclosion were measured, the resistance of heart leaves, maize silks and seeds of transgenic corn CM8101 with Cry1 Ab-ma gene to the 1 st and 2 nd instar larvae of S. frugiperda were analyzed. The results showed that the 1 st instar larvae were completely killed by the heart leaves, and the 2 nd instar larvae could survive with survival rate10.0%, but could not complete the life cycle of pupation and eclosion. The most 1 st instar larvae with survival rate of 3.3% could be killed by maize silks and their life cycles were interrupted, however, it was not lethal to 2 nd instar larvae with survival rate of 73.3%, and pupation rate was 20.0%, eclosion rate was 13.3%. The 1 st instar larvae were completely killed by seeds, and the 2 nd instar larvae could survive with survival rate 13.3%, had less pupae with pupation rate of 3.3%, and had less pupae with eclosion rate of 3.3%. The results indicated that the transgenic maize CM8101 with Cry1 Ab-ma gene had high control efficacy against the 1 st and 2 nd instar larvae of fall armyworm, and could effectively block their life cycles and reduce the pest damage.

关 键 词：草地贪夜蛾 转Cry1Ab-ma基因玉米CM8101 抗虫性 室内生测 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]