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作 者:A.Pascual M.Pauletto M.Giantin G.Radaelli C.Ballarin M.Birolo C.Zomeño M.Dacasto M.Bortoletti M.Vascellari G.Xiccato A.Trocino
机构地区:[1]Department of Comparative Biomedicine and Food Science(BCA),University of Padova,Viale dell’Università16,I-35020 Legnaro,Padova,Italy [2]department of agronomy,food,natural resources,animal,and environment(dafnae),university of padova,viale dell’università16,i-35020 legnaro,padova,italy [3]histopathology department,istituto zooprofilattico sperimentale delle venezie,viale dell’università10,i-35020 legnaro,padova,italy
出 处:《Journal of Animal Science and Biotechnology》2020年第3期736-746,共11页畜牧与生物技术杂志(英文版)
基 金:The project was funded by Lesaffre(C26C18000320007).
摘 要:Background:The dietary supplementation of yeast cell wall extracts(YCW)has been found to reduce pathogenic bacteria load,promote immunoglobulin production,prevent diseases by pro-inflammatory responses,and alter gut microbiota composition.This study evaluated growth and slaughter results,health,gut morphology,immune status and gut transcriptome of 576 male chickens fed two diets,i.e.C(control)or Y(with 250–500 g/t of YCW fractions according to the growth period).At 21 and 42 d the jejunum of 12 chickens per diet were sampled and stained with hematoxylin/eosin for morphometric evaluation,with Alcian-PAS for goblet cells,and antibodies against CD3+intraepithelial T-cells and CD45+intraepithelial leukocytes.The jejunum sampled at 42 d were also used for wholetranscriptome profiling.Results:Dietary YCW supplementation did not affect final live weight,whereas it decreased feed intake(114 to 111 g/d;P≤0.10)and improved feed conversion(1.74 to 1.70;P≤0.01).Regarding the gut,YCW supplementation tended to increase villi height(P=0.07);it also increased the number of goblet cells and reduced the density of CD45+cells compared to diet C(P<0.001).In the gut transcriptome,four genes were expressed more in broilers fed diet Y compared to diet C,i.e.cytochrome P450,family 2,subfamily C,polypeptide 23b(CYP2C23B),tetratricopeptide repeat domain 9(TTC9),basic helix-loop-helix family member e41(BHLHE41),and the metalloreductase STEAP4.Only one gene set(HES_PATHWAY)was significantly enriched among the transcripts more expressed in broilers fed diet Y.However,a total of 41 gene sets were significantly over-represented among genes up-regulated in control broilers.Notably,several enriched gene sets are implicated in immune functions and related to NF-κB signaling,apoptosis,and interferon signals.Conclusions:The dietary YCW supplementation improved broiler growth performance,increased gut glycoconjugate secretion and reduced the inflammatory status together with differences in the gut transcriptome,which can be considered useful
关 键 词:Gut morphometry IMMUNO-HISTOCHEMISTRY Saccharomyces cerevisiae Transcriptomic analysis
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