Effects of adding sodium dichloroacetate to low-protein diets on nitrogen balance and amino acid metabolism in the portaldrained viscera and liver of pigs  

作　　者：Weizhong Sun Yunxia Li Zhiru Tang Huiyuan Chen Ke Wan Rui An Liuting Wu Zhihong Sun 

机构地区：[1]Laboratory for Bio-feed and Molecular Nutrition,College of Animal Science and Technology,Southwest University,Chongqing 400715,People’s Republic of China [2]Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu 611130,People’s Republic of China

出　　处：《Journal of Animal Science and Biotechnology》2020年第3期884-895,共12页畜牧与生物技术杂志（英文版）

基　　金：This study was funded by grants from the National Natural Science Foundation of China(31872370,31670123);the Fundamental Research Funds for the Central Universities(XDJK2019B014,XDJK2013C097);the Natural Science Foundation Project of CQ CSTC(cstc2018jcyjAX0025).

摘　　要：Background:Identifying regulatory measures to promote glucose oxidative metabolism while simultaneously reducing amino acid oxidative metabolism is one of the foremost challenges in formulating low-protein(LP)diets designed to reduce the excretion of nitrogen-containing substances known to be potential pollutants.In this study,we investigated the effects of adding sodium dichloroacetate(DCA)to a LP diet on nitrogen balance and amino acid metabolism in the portal-drained viscera(PDV)and liver of pigs.To measure nitrogen balance,18 barrows(40±1.0 kg)were fed one of three diets(n=6 per group):18%crude protein(CP,control),13.5%CP(LP),and 13.5%CP+100 mg DCA/kg dry matter(LP-DCA).To measure amino acid metabolism in the PDV and liver,15 barrows(40±1.0 kg)were randomly assigned to one of the three diets(n=5 per group).Four essential amino acids(Lys,Met,Thr,and Trp)were added to the LP diets such that these had amino acid levels comparable to those of the control diet.Results:The LP-DCA diet reduced nitrogen excretion in pigs relative to that of pigs fed the control diet(P<0.05),without any negative effects on nitrogen retention(P>0.05).There were no differences between the control and LP-DCA groups with respect to amino acid supply to the liver and extra-hepatic tissues in pigs(P>0.05).The net release of ammonia into the portal vein and production rate of urea in the liver of pigs fed the LP-DCA diet was reduced relative to that of pigs fed the control and LP diets(P<0.05).Conclusion:The results indicated that addition of DCA to a LP diet can efficiently reduce nitrogen excretion in pigs and maximize the supply of amino acids to the liver and extra-hepatic tissues.

关 键 词：Amino acid metabolism Low-protein diet Nitrogen excretion PIG Sodium dichloroacetate 

分 类 号：S828.5[农业科学—畜牧学]