植物生长调节剂对金线莲玻璃化苗复壮与增殖的影响  

Effect of Plant Growth Regulators on Rejuvenation and Proliferation of Vitrified Seedlings of Anoectochilus roxburghii

作　　者：刘芳 唐映红 李春艳 巩媛 毛凯权 陈建荣 LIU Fang;TANG Yinghong;LI Chunyan;GONG Yuan;MAO Kaiquan;CHEN Jianrong(College of Biological and Environmental Engineering,Changsha University,Changsha 410022,China;College of Life and Environmental Sciences,Hunan University of Arts and Science,Changde 415000,China)

机构地区：[1]长沙学院生物与环境工程学院,湖南长沙410022 [2]湖南文理学院生命与环境科学学院,湖南常德415000

出　　处：《湖南生态科学学报》2020年第3期15-20,共6页Journal of Hunan Ecological Science

基　　金：湖南省自然科学基金项目(2014CK4018);湖南省长沙市科技计划项目(K1205040-31)。

摘　　要：金线莲市场应用前景广阔,但资源短缺,利用组织培养技术进行金线莲的快速繁殖,能有效解决金线莲繁殖周期长、市场供应不足等问题,对保护野生金线莲资源具有重要意义.但在试验过程中常常由于植物生长调节剂配比或培养条件不合理导致试验材料产生玻璃化导致试验材料浪费.针对这种情况,本试验在多因素筛选后的基础上,采用单一变量的方法,研究了不同的植物调节剂配比对玻璃化苗复壮的影响.结果显示,不仅玻璃化苗复壮并且得到了较好的增殖及原球茎诱导效果.金线莲玻璃化苗复壮最佳配比为:MS+2.0 mg/L 6-BA+1.0 mg/L NAA;最佳增殖的配比为:MS+2.0 mg/L 6-BA+0.01 mg/L NAA,培养条件为温度24℃、光照强度2000 lx、光照12 h/d.The market prospect of Anoectochilus roxburghii is broad,but its resources are in short supply.The tissue culture technology is used for rapid propagation of Anoectochilus roxburghii,which can effectively solve the problems such as long breeding cycle of Anoectochilus roxburghii and undersupply of the market.It is of great significance to the protection of wild Anoectochilus resources.But in the process of test,vitrification of test materials are caused often by unreasonable plant growth regulator proportions or culture conditions,which leads to waste of test materials.So this experiment is based on multi factorafter screening,using a single variable,to study the effects of proportion of different plant growth regulators on recovery of vitrified seedlings.The results showed that:Vitrified seedlings were resuscitated and better pro-liferation and protocorm induction were obtained.The optimal proportion for recovery of Anoectochilus roxburghii was MS+2.0 mg/L 6-BA+1.0 mg/L NAA;The optimal proportion of proliferation was MS+2.0 mg/L 6-BA+0.01 mg/L NAA,with the culture conditions of 24℃,light intensity,light 12 h/d 2000 lx.

关 键 词：组织培养 金线莲 玻璃化 植物生长调节剂 玻璃化苗复壮 

分 类 号：Q945.52[生物学—植物学]