Lasiodiplodia pseudotheobromae causes stem canker of Chinese hackberry in China  

作　　者：Lu Liang Huan Li Lifeng Zhou Fengmao Chen 

机构地区：[1]Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,People’s Republic of China

出　　处：《Journal of Forestry Research》2020年第6期2571-2580,共10页林业研究（英文版）

摘　　要：Chinese hackberry(Celtis sinensis Pers.)is an adaptable species widely growing in southern China.The symptoms of canker on stems of seedlings were discovered mid-July 2017 in Shuyang,Jiangsu Province.The diseased portions of the stems were dark brown due to discoloured xylem.Some seedlings showed symptoms of wilting,leaf fall,twig dieback,and tissue discolouration.The outbreak period was concentrated in July and August,suggesting that the disease spread during summer months.Possible fungal causal agents were isolated from naturally infected canker tissue and discoloured xylem.The isolate from xylem tissue with a high frequency(>50%)was named Ls7 type.Pathogenicity tests were carried out on 4-yearold seedlings.The symptoms of canker began to develop 20 days after inoculation with Ls7 isolate and by day 35,there were dark,enlarged longitudinal lesions.A phylogenetic tree of the isolate was developed using the internal transcribed spacer,elongation factor-1α(tef1-α),β-tubulin gene(TUB)and RNA polymerase II subunit primer genes(RPB2).Based on morphological features and phylogenetic information,the pathogen was identifi ed as Lasiodiplodia pseudotheobromae.This is the fi rst report of L.pseudotheobromae causing canker on Chinese hackberry stems in China.

关 键 词：BOTRYOSPHAERIACEAE Plant pathogen Tree disease 

分 类 号：S435.671[农业科学—农业昆虫与害虫防治]