High-throughput sequencing analysis of the regulation of intestinal flora in giant pandas with indigestion using a probiotic agent LyPB  

作　　者：Yu Zhang Zhi Zhang Jianzhang Ma Bo Luo Guiquan Zhang Guocai Zhang Kexin Yang Gang Wei 

机构地区：[1]School of Forestry,Northeast Forestry University,Harbin 150040,People’s Republic of China [2]China Conservation and Research Center for Giant Panda,Ya’an,People’s Republic of China [3]School of Computer Science and Technology,Harbin Institute of Technology,Harbin,People’s Republic of China

出　　处：《Journal of Forestry Research》2020年第6期2589-2595,共7页林业研究（英文版）

摘　　要：This study aimed to investigate the eff ect of LyPB on the intestinal microfl ora of giant pandas with indigestion,using high-throughput sequencing(HTS)technology.The species distribution and microfl oral density and diversity before and after administration of the LyPB probiotic agent were analyzed.LyPB evidently has the ability to adjust the fl oral imbalance in the panda’s intestine.To test the eff ects of LyPB on the microfl ora of the panda gut,fecal samples were taken from a healthy giant panda(Anan)without administration of LyPB and from a dyspeptic giant panda Yangyang before and after LyPB administration.Compared with the sample obtained from healthy Anan(anan-c)and that obtained from dyspeptic Yangyang before LyPB administration(yangyang1),the sample taken from Yangyang(yangyang2)after LyPB administration displayed a signifi cant increase in the operational taxonomic unit index.An increase in the Chao index indicated an increase in the microfl oral richness,while an increase in the Shannon index indicated an increase in microfl oral diversity.At phylum and genus levels,a signifi cant increase was observed in the density of probiotic bacteria of phylum fi rmicutes,genus Streptococcus,while a drastic reduction in the density of Escherichia coli/Escherichia coli Shigella/bacteria of genus Shigella was observed.Data obtained in this study shows that LyPB preparations successfully improve the microbial structure within the panda’s intestinal canal by signifi cantly increasing the eff ective microbial community and decreasing the number of pathogenic microbes.

关 键 词：High-throughput sequencing Intestinal fl ora PANDAS Indigestion LyPB 

分 类 号：Q938[生物学—微生物学]