葆肾合剂HPLC指纹图谱研究  

Study on the HPLC Fingerprint Chromatogram of Baoshen Mixtures

作　　者：冯子芳[1] 袁晓航[1] 陈晓伟 胡敏敏[1] 费倩倩 费逸明 Feng Zifang;Yuan Xiaohang;Chen Xiaowei;Hu Minmin;Fei Qianqian;Fei Yiming(Wuxi Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine,Jiangsu Wuxi 214000,China)

机构地区：[1]南京中医药大学附属无锡市中医医院,江苏无锡214000

出　　处：《中国药师》2020年第9期1750-1754,共5页China Pharmacist

基　　金：无锡市卫计委中医药科研项目(编号:ZYZL201802)。

摘　　要：目的:建立葆肾合剂的HPLC指纹图谱,以控制该中药复方制剂的质量。方法:采用Waters XBridge C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.2%磷酸水溶液(B)梯度洗脱,体积流量为1.0 ml·min^-1,检测波长为326 nm(0~26 min)和254 nm(26~75 min),柱温为30℃,进样体积为10μl。结果:建立了10批葆肾合剂的HPLC指纹图谱,相似度均大于0.95,标定了14个共有峰,指认了其中4个色谱峰,分别为绿原酸、薏苡素、鞣花酸和毛蕊异黄酮葡萄糖苷,并进行了共有峰的原药材归属。结论:建立的方法稳定可靠、简便、重复性好,可用于葆肾合剂的质量控制。Objective:To establish the HPLC fingerprint of Baoshen mixtures to control the quality of the Chinese compound preparation.Methods:Using a Waters XBridge C18 column(250 mm×4.6 mm,5μm),the mobile phase consisted of acetonitrile(A)-0.2%phosphoric acid aqueous solution(B)with gradient elution at the flow rate of 1.0 ml·min^-1.The detection wavelengths were 326 nm(0-26 min)and 254 nm(26-75 min),the column temperature was 30℃,and the sample volume was 10μl.Results:The HPLC fingerprints of 10 batches of Baoshen mixtures were established,the similarity was more than 0.95,14 common peaks were demarcated,and 4 of them were identified,which were chlorogenic acid,coixol,ellagic acid and isoflavone glucoside,and the common peaks of the original medicinal materials were assigned.Conclusion:The established method is stable,reliable,simple and reproducible,and can be used for the quality control of Baoshen mixtures.

关 键 词：葆肾合剂 高效液相色谱法 指纹图谱 质量控制 

分 类 号：TQ460.72[医药卫生—药物分析学]