超短波对急性肺损伤大鼠炎症反应的疗效及其机制  

Effects of Ultrashort Wave on Inflammatory of Acute Lung Injury and Related Signaling Pathway in Rats

作　　者：周君[1,2,3] 刘丹妮 王丽琼 王谦 李懿[1,2] 屈萌艰 刘静[3] 曾亚华 何成奇 ZHOU Jun;LIU Dan-ni;WANG Li-qiong;WANG Qian;LI Yi;QU Meng-jian;LIU Jing;ZENG Yahua;HE Cheng-qi(Department of Rehabilitation,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Rehabilitation Key Laboratory of Sichuan Province,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,China;Department of Rehabilitation,the First Affiliated Hospital of University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]四川大学华西医院康复医学科,四川成都市610041 [2]康复医学四川省重点实验室,四川成都市610041 [3]南华大学附属第一医院康复医学科,湖南衡阳市421001

出　　处：《中国康复理论与实践》2020年第10期1176-1181,共6页Chinese Journal of Rehabilitation Theory and Practice

基　　金：湖南省自然科学基金项目(No.2019JJ50544);衡阳市科技局项目(No.HKF201947209);南华大学附属第一医院亚专科重点项目(No.2017ZD007)。

摘　　要：目的观察超短波疗法对脂多糖诱导的大鼠急性肺损伤炎症反应的效果,以及对核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)信号通路的影响。方法3月龄Sprague-Dawley雄性大鼠24只随机分为对照组、急性肺损伤组和超短波组,每组8只。后两组气管内滴注脂多糖复制急性肺损伤模型,超短波组在造模后即刻、4 h、8 h予超短波干预15 min。造模后24 h处死动物,测量肺组织湿/干重比(W/D);HE染色评估损伤程度;ELISA检测白细胞介素(IL)-1β和IL-18水平;逆转录聚合酶链反应和Western blotting检测NLRP3、caspase-1和IL-1βmRNA和蛋白表达。结果急性肺损伤组肺组织W/D较对照组升高(P<0.05),超短波组较急性肺损伤组有降低趋势,但无显著性差异(P>0.05)。急性肺损伤组肺损伤评分较对照组增高(P<0.05),超短波组较急性肺损伤组降低(P<0.05)。急性肺损伤组血清IL-1β和IL-18水平均较对照组升高(P<0.05),超短波组较急性肺损伤组降低(P<0.05)。NLRP3、caspase-1和IL-1βmRNA和蛋白表达,急性肺损伤组均较对照组增高(P<0.05),超短波组较急性肺损伤组降低(P<0.05)。结论超短波疗法能抑制大鼠急性肺损伤的炎症反应,可能与抑制NLRP3/caspase-1/IL-1β信号通路有关。Objective To observe the effects of ultrashort wave(USW)on lipopolysaccharide(LPS)induced acute lung injury(ALI)and nucleotide-binding oligomerization domain like receptor protein 3(NLRP3)signaling pathway in rats.Methods Twenty-four three-month-old male Sprague-Dawley rats were randomly divided into control group(n=8),ALI group(n=8)and USW group(n=8).The ALI and USW groups were instilled with LPS to induce ALI,and the USW group was treated with ultrashort wave 0,four and eight hours after instillation,15 minutes a time.Twenty-four hours after instillation,the lung tissue of the rats was measured the wet/dry mass ratio(W/D),and observed under HE staining.Serum levels of interleukin(IL)-1βand IL-18 were detected with ELISA.The mRNA and protein expression of NLRP3,caspase-1 and IL-1βin the lung tissue were detected with reverse transcription polymerase chain reaction and Western blotting,respectively.Results W/D increased in ALI group compared with that of the control group(P<0.05),and it decreased in USW group without significance compared with that of ALI group(P>0.05).Lung injury score increased in ALI group compared with that of the control group(P<0.05),and it decreased in USW group compared with that of ALI group(P<0.05);as well as the serum IL-1βand IL-18,and mRNA and protein expression of NLRP3,caspase-1 and IL-1β.Conclusion USW can alleviate the inflammatory of acute lung injury,which may associate with inhibiting of NLRP3 signaling pathway.

关 键 词：急性肺损伤 超短波 炎症 核苷酸结合寡聚化结构域样受体蛋白3 信号通路 大鼠 

分 类 号：R563.1[医药卫生—呼吸系统]