miR-122-5p靶向切除修复交叉互补1对卵巢癌细胞生物学行为的影响  

Effect of miR-122-5p targeted excision repair cross complementation 1 on the biological behavior of ovarian cancer cells

作　　者：苗培田 佟仲生[2] 王磊[3] MIAO Pei-tian;TONG Zhong-sheng;WANG Lei(Department of Oncology,Ninghe District Hospital of Tianjin,Tianjin 301500,China;Department of Breast Medicine,Cancer Hospital Affiliated to Tianjin Medical University,Tianjin 301500,China;Hospital Affiliated to Chengde Medical College,Chengde 067000,Hebei Province,China)

机构地区：[1]天津市宁河区医院肿瘤科,天津301500 [2]天津医科大学附属肿瘤医院乳腺内科,天津301500 [3]承德医学院附属医院,河北承德067000

出　　处：《中国临床药理学杂志》2020年第18期2856-2860,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河北省医学科学研究重点课题计划基金资助项目(20170889)。

摘　　要：目的研究miR-122-5p对卵巢癌细胞增殖、迁移、侵袭和凋亡的影响及潜在的分子机制。方法选取确诊的卵巢癌患者的卵巢癌组织标本和卵巢癌旁正常组织各46例。以实时定量-PCR法检测miR-122-5p在卵巢癌组织、癌旁组织的表达。将卵巢癌细胞HO8910分为4组:第1转染组(转染miR-NC)、第2转染组(转染miR-122-5p)、第3转染组(转染miR-122-5p+pcDNA3.1)和第4转染组[转染miR-122-5p+pcDNA3.1-切除修复交叉互补1(ERCC1)]。以MTT法、Transwell实验和流式细胞术分别测定HO8910细胞增殖活性、细胞迁移和侵袭能力及细胞凋亡率,双荧光素酶报告系统验证miR-122-5p与ERCC1的结合关系。结果癌旁组织、卵巢癌组织的miR-122-5p表达量分别为0.58±0.05和0.13±0.01,两者相比差异有统计学意义(P<0.05)。第1转染组、第2转染组、第3转染组和第4转染组的细胞活性分别为0.79±0.08,0.38±0.04,0.36±0.03和0.57±0.05;这4组的细胞迁移数分别为116.45±11.36,45.23±4.51,45.00±4.37和83.00±8.64;这4组的细胞侵袭数分别为109.02±11.13,41.15±4.13,40.00±4.16和76.00±7.92;这4组的细胞凋亡率分别为(8.25±0.84)%,(24.61±2.44)%,(24.55±2.51)%和(13.67±1.38)%。上述指标:第2转染组与第1转染组相比,或第4转染组与第3转染组相比,差异均有统计学意义(均P<0.05)。第1转染组和第2转染组WT-ercc1的荧光素酶活性分别为1.04±0.10和0.28±0.03,2组相比差异有统计学意义(P<0.05)。结论miR-122-5p通过靶向ERCC1抑制HO8910细胞的增殖、迁移和侵袭,促进细胞凋亡。Objective To investigate the effects of miR-122-5 p on proliferation,migration,invasion and apoptosis of ovarian cancer cells and the potential mechanism.Methods Forty-six cases of ovarian cancer tissue specimens and normal tissues adjacent to ovarian cancer were selected.MiR-122-5 p expression in ovarian cancer tissues and adjacent tissues were detected by real-time quantitative-PCR.The ovarian cancer cell HO8910 was divided into four groups:transfection-1 group(transfected with miR-NC),transfection-2 group(transfected with miR-122-5 p),transfection-3 group(transfected with miR-122-5 p and pcDNA3.1),and transfection-4 group[transfected with miR-122-5 p and pcDNA3.1-excision repair cross-complementation 1(ERCC1)].MTT assay,Transwell experiment and flow cytometry were used to respectively measure the proliferation activity,cell migration and invasion ability and cell apoptosis rate of HO8910 cells.The targeting relationship between miR-122-5 p and ERCC1 was verified by double luciferase reporting system.Results The expression levels of miR-122-5 p in paracancerous tissues and ovarian cancer tissues were 0.58±0.05 and 0.13±0.01,respectively;the difference between ovarian cancer tissues and adjacent tissues was statistically significant(P<0.05).The cell activities in transfection-1 group,transfection-2 group,transfection-3 group,and transfection-4 group were 0.79±0.08,0.38±0.04,0.36±0.03,and 0.57±0.05;the cell migration numbers in the four groups were 116.45±11.36,45.23±4.51,45.00±4.37,83.00±8.64,respectively;the cell invasion numbers in the four groups were109.02±11.13,41.15±4.13,40.00±4.16,76.00±7.92;the cell apoptosis rates in the four groups were(8.25±0.84)%,(24.61±2.44)%,(24.55±2.51)%,(13.67±1.38)%;comparison between transfection-2 group and transfection-1 group,or between transfection-4 group and transfection-3 group,the differences of the factors were statistically significant(all P<0.05).The luciferase activities of WT-ercc1 in transfection-1 group and transfection-2 group were 1.04±0.10,0

关 键 词：卵巢癌 微小核糖核酸122-5p 切除修复交叉互补1 增殖 迁移 侵袭 凋亡 

分 类 号：R97[医药卫生—药品]