右美托咪定对抗炎性模型中巨噬细胞迁移能力与炎性因子释放的研究  

Research of dexmedetomidine on the migration of macrophages and the release of inflammatory factors in the anti-inflammatory model

作　　者：解翔彬 朱平峰 舒化青[2] XIE Xiang-bin;ZHU Ping-feng;SHU Hua-qing(Department of Anesthesiology,Sanya People’s Hospital,Sanya 572000,Hainan Province,China;Institute of Anesthesiology and Critical Care Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China)

机构地区：[1]三亚市人民医院麻醉科,海南三亚572000 [2]华中科技大学同济医学院附属协和医院麻醉与危重医学研究所,湖北武汉430022

出　　处：《中国临床药理学杂志》2020年第18期2883-2886,共4页The Chinese Journal of Clinical Pharmacology

基　　金：海南省科技厅课题基金资助项目(ZDYF2018126)。

摘　　要：目的分析右美托咪定(Dex)对脂多糖(LPS)诱导炎性模型中巨噬细胞迁移能力与炎性因子释放的影响。方法以LPS刺激小鼠腹腔巨噬细胞建立炎性模型。实验随机分为8组:A组加入新的培养液正常培养;B组加入含10μg·L^-1 Dex的培养液培养24 h;C组加入含1 mg·L^-1 LPS的培养液培养24 h;D组加入含10μg·L^-1 Dex与1 mg·L^-1 LPS的培养液培养24 h;E组转染肝细胞Janus蛋白激酶2(JAK-2)siRNA 48 h后,加入含LPS的培养液培养24 h;F组转染信号转导转录激活子3(STAT-3)siRNA 48 h后,加入含LPS的培养液培养24 h;G组转染JAK-2 siRNA 48 h后,加入含Dex与LPS的培养液培养24 h;H组转染STAT-3 siRNA 48 h后,含Dex与LPS的培养液培养24 h。检测各组细胞的迁移能力和炎性因子分泌水平。结果A,C,D,E,F,G和H组的肿瘤坏死因子-α分别为(60.87±2.13),(266.31±43.11),(138.29±21.22),(163.20±25.23),(154.13±17.28),(95.27±9.18)和(90.17±9.20)ng·L^-1,白细胞介素-6(IL-6)分别为(42.23±3.45),(168.65±37.14),(96.57±19.57),(116.37±11.89),(109.18±10.37),(69.82±3.78)和(67.13±6.12)ng·L^-1,IL^-1β分别为(48.72±6.13),(149.35±20.17),(92.35±10.78),(89.31±15.12),(99.13±14.67),(54.39±9.71)和(62.64±3.47)ng·L^-1,C组的上述指标与A,D,E,F,G,H组比较,E组的上述指标与G组比较,以及F组的上述指标与H组比较,差异均有统计学意义(均P<0.05)。A,C,D,E,F,G和H组的细胞迁移数量分别为(183.23±19.26),(634.29±10.13),(378.09±16.27),(620.19±15.18),(619.37±14.28),(369.33±11.37)和(371.26±9.66)个,C,E,F组的迁移细胞数量与A组比较,D,G,H组的迁移细胞数量与C组比较,差异均有统计学意义(均P<0.05)。结论Dex可抑制LPS诱导的巨噬细胞迁移能力与炎性反应,其抗炎作用途径可能与抑制JAK-2/STAT-3信号通路有关。Objective To analyze the effect of dexmedetomidine(Dex)on the lipopolysaccharide(LPS)-induced macrophage migration and the release of inflammatory inflammatory factors.Methods The inflammatory model was established by stimulating mouse peritoneal macrophages with LPS.The experiment was randomly divided into 8 groups:A group was added with new culture medium for normal culture;B group was added with a culture medium containing 10μg·L^-1 Dex for 24 h;C group was added with a culture medium containing 1 mg·L^-1 LPS for 24 h;D group was added with culture medium containing 10μg·L^-1 Dex and 1 mg·L^-1 LPS for 24 h;E group was transfected with Janus protein kinase 2(JAK-2)siRNA for 48 h,and then added culture medium containing LPS was cultured for 24 h;F group was transfected with signal transduction transcription activator 3(STAT-3)siRNA for 48 h,and culture medium containing LPS was added for 24 h;G group was transfected with JAK-2 siRNA for 48 h,and culture medium containing Dex and LPS was added for 24 h;H group was transfected with STAT-3 siRNA for 48 h,and culture medium containing Dex and LPS was cultured for 24 h.The ability of cell migration and the secretion of inflammatory factors in each group were detected.Results The tumor necrosis factor-αin A,C,D,E,F,G and H groups were(60.87±2.13),(266.31±43.11),(138.29±21.22),(163.20±25.23),(154.13±17.28),(95.27±9.18)and(90.17±9.20)ng·L^-1,interleukin-6(IL-6)were(42.23±3.45),(168.65±37.14),(96.57±19.57),(116.37±11.89),(109.18±10.37),(69.82±3.78)and(67.13±6.12)ng·L^-1,IL^-1βwere(48.72±6.13),(149.35±20.17),(92.35±10.78),(89.31±15.12),(99.13±14.67),(54.39±9.71)and(62.64±3.47)ng·L^-1.The above-mentioned indexes in C group were compared with those in A,D,E,F,G,H groups,the above-mentioned indexes in E group were compared with those in G group,and the above-mentioned indexes in F group were compared with those in H group,with statistically significant differences(all P<0.05).The number of cell migration in A,C,D,E,F,G and H groups was(183.23�

关 键 词：右美托咪定 巨噬细胞 脂多糖 炎性因子 迁移能力 

分 类 号：R971.2[医药卫生—药品]