Mg-Zn-Y-Nd合金胆囊夹在兔体内的降解行为及生物相容性  

Degradation behavior and biocompatibility of Mg-Zn-Y-Nd alloy gallbladder in rabbits

作　　者：许凯 王战会[2] 关绍康[3] 张兵兵[2] 段廷贺[2] 刘少朋 孙宗斌[2] 郑秋霞 XU Kai;WANG Zhanhui;GUAN Shaokang;ZHANG Bingbing;DUAN Tinghe;LIU Shaopeng;SUN Zongbin;ZHENG Qiuxia(Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Department of Hepatobiliary Surgery,Luoyang Central Hospital Affiliated to Zhengzhou University,Luoyang 471009,Henan Province,China;School of Materials Science and Engineering,Zhengzhou University,Zhengzhou 450000,Henan Province,China)

机构地区：[1]新乡医学院,河南新乡453003 [2]郑州大学附属洛阳市中心医院肝胆外科,河南洛阳471009 [3]郑州大学材料科学与工程学院,河南郑州450000

出　　处：《新乡医学院学报》2020年第9期813-817,823,共6页Journal of Xinxiang Medical University

基　　金：国家自然科学基金联合基金项目(编号:U1404825)。

摘　　要：目的探讨Mg-Zn-Y-Nd合金胆囊夹在兔体内的降解行为及生物相容性。方法将36只兔随机分为裸合金夹组(A组)、植酸涂层合金夹组(B组)、MAO/植酸涂层合金夹组(C组)和空白对照组(D组),每组9只。D组兔不进行手术;其余各组兔行开腹手术,取灭菌处理后的不同涂层Mg-Zn-Y-Nd合金夹夹闭选取的胆囊壁组织。观察各组兔术后一般情况;检测各组兔手术前后血清镁离子、钙离子浓度及肝肾功能;术后7、14、21 d,采用X射线衍射观察各手术组兔合金夹降解情况;术后7、14、21 d分批处死各手术组兔并取出合金夹,通过扫描电镜观察合金夹降解情况。结果术后各组兔生命体征平稳,未出现并发症。X射线衍射和扫描电镜结果显示,随着术后时间的延长,A组兔合金夹表面结构缺损最为明显;B组兔合金夹表面裂纹加深;C组兔合金夹表面仅可见轻微裂纹,结构完整。手术前后各时间点4组兔血清镁离子、钙离子浓度及肝、肾功能指标比较差异均无统计学意义(P>0.05)。结论Mg-Zn-Y-Nd合金夹在兔体内生物相容性良好,且生物学毒性小,安全性高。MAO/植酸涂层合金夹在兔体内降解速率明显低于裸合金夹,且其生物相容性更好。Objective To investigate the degradation behavior and biocompatibility of Mg-Zn-Y-Nd alloy gallbladder in rabbits.Methods Thirty-six rabbits were randomly divided into bare metal alloy clamp group(group A),phytic acid coated alloy clip group(group B),microarc oxidation(MAO)/phytic acid coated alloy clip group(group C)and blank control group(group D),with 9 rabbits in each group.Rabbits in group D were not given operation;the rabbits in the other groups were given open surgery and the gallbladder wall tissues were clamped with sterilized Mg-Zn-Y-Nd alloy clamp with different coating.The general situation of rabbits in the operation group was observed after operation.The serum levels of magnesium ion,calcium ion and indexes of liver function,kidney function were measured.The degradation of alloy clamp in each operation group was observe d by X-ray diffraction at 7,14,21 days after operation;the rabbits in each operation group were sacrificed and the alloy clamp was removed at 7,14,21 days after operation to observe the degradation of the alloy clamp by scanning electron microscope.Results The vital signs of rabbits in each group were stable and no complications occurred after operation.The results of X-ray diffraction and scanning electron microscope showed that with the prolongation of postoperative time,the structural defects on the surface of gold clip in group A was the most obvious;the crack of gold clip in group B deepened;only slight cracks could be seen on the surface of gold clip and the structure of gold clip was complete in group C.There was no significant difference in serum levels of magnesium ion,calcium ion and the indexes of liver function,renal function among the four groups at different time points before and after operation(P>0.05).Conclusion Mg-Zn-Y-Nd alloy clips have good biocompatibility,low biological toxicity and high safety in rabbits.The degradation rate of MAO/phytic acid coated alloy in rabbits is lower than that of bare metal alloy clamp,and its biocompatibility is better.

关 键 词：镁合金 生物相容性 降解 胆囊夹 

分 类 号：R318.08[医药卫生—生物医学工程]