丙泊酚对创伤后应激障碍大鼠恐惧记忆的影响及可能机制  

Effect of propofol on fear memory of rats with post-traumatic stress disorder and its possible mechanism

作　　者：张晓冉[1] 马闻苛 樊腾[1] 田建民[1] 高宝琴[1] 张永强[1] ZHANG Xiaoran;MA Wenke;FAN Teng;TIAN Jianmin;GAO Baoqin;ZHANG Yongqiang(Department of Anesthesiology,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China)

机构地区：[1]新乡医学院第一附属医院麻醉科,河南卫辉453100

出　　处：《新乡医学院学报》2020年第9期824-829,共6页Journal of Xinxiang Medical University

基　　金：河南省卫生科技创新型人才工程中青年科技创新人才资助项目(编号:20114155)。

摘　　要：目的探讨丙泊酚对创伤后应激障碍(PTSD)大鼠恐惧记忆的影响及可能机制。方法将60只雄性Sprague Dawley大鼠随机分为对照组、消退对照组、消退训练组、消退训练+丙泊酚低剂量组、消退训练+丙泊酚中剂量组和消退训练+丙泊酚高剂量组,每组10只。对照组大鼠进行标准饲养,不给予任何处理;其余5组大鼠采用单一连续应激法建立PTSD模型。造模成功后第9~14天,消退对照组大鼠不给予任何处理,消退训练+丙泊酚低剂量组大鼠每日给予消退训练并腹腔注射0.2 mg·kg^-1丙泊酚,消退训练+丙泊酚中剂量组大鼠每日给予消退训练并腹腔注射0.6 mg·kg^-1丙泊酚,消退训练+丙泊酚高剂量组大鼠每日给予消退训练并腹腔注射1.0 mg·kg^-1丙泊酚,消退训练组大鼠每日给予消退训练并腹腔注射等量生理盐水,连续6 d。采用高架十字迷宫实验测各组大鼠焦虑状态,条件性恐惧记忆实验检测各组大鼠恐惧记忆,酶联免疫吸附试验检测各组大鼠血清皮质酮水平,Western blotting法检测各组大鼠海马组织中脑源性神经营养因子(BDNF)及其特异性受体TrkB蛋白的表达水平。结果消退对照组、消退训练组、消退训练+丙泊酚低剂量组、消退训练+丙泊酚中剂量组和消退训练+丙泊酚高剂量组大鼠僵直停留时间均显著长于对照组(P<0.05);消退训练组、消退训练+丙泊酚低剂量组、消退训练+丙泊酚中剂量组和消退训练+丙泊酚高剂量组大鼠僵直停留时间均显著短于消退对照组(P<0.05);消退训练+丙泊酚低剂量组、消退训练+丙泊酚中剂量组和消退训练+丙泊酚高剂量组大鼠僵直停留时间均显著短于消退训练组(P<0.05);不同剂量丙泊酚组大鼠僵直停留时间随丙泊酚剂量的增加逐渐缩短,两两比较差异均有统计学意义(P<0.05)。消退对照组、消退训练组、消退训练+丙泊酚低剂量组大鼠开臂停留时间和开臂进入次数显著低�Objective To investigate the effect of propofol on fear memory of post-traumatic stress disorder(PTSD)rats and its possible mechanism.Methods Sixty male Sprague Dawley rats were randomly divided into control group,regression control group,regression training group,regression training+low dose propofol group,regression training+medium dose propofol group and regression training+high dose propofol group,with 10 rats in each group.The rats in the control group were fed with standard diet without any treatment,and the rats in the other five groups were made the PTSD model by single continuous stress method.At the 9-14 days after successful modeling,the rats in the regression control group were not given any treatment;the rats in the regression training+low dose propofol group were given regression training and intraperitoneal injection of 0.2 mg·kg^-1 propofol every day for 6 days;the rats in the regression training+medium dose propofol group were given regression training and intraperitoneal injection of 0.6 mg·kg^-1 every day for 6 days;the rats in the regression training+high dose propofol group were given regression training and intraperitoneal injection of 1.0 mg·kg^-1 propofol every day for 6 days;the rats in the regression training group were given regression training and intraperitoneal injection of the same amount of normal saline for 6 days.The anxiety state of all rats was measured by elevated plus-maze test;the fear memory of all rats was measured by conditional fear memory test.Serum corticosterone levels of all rats were measured by enzyme linked immunosorbent assay.The expressions of brain-derived neurotrophic factor(BDNF)and its specific receptor TrkB protein were detected by Western blotting.Results The stiffness stay time of rats in the regression control group,regression training group,regression training+low dose propofol group,regression training+medium dose propofol group and regression training+high dose propofol group were significantly longer than those in the control group(P<0.05).The sti

关 键 词：创伤后应激障碍 恐惧记忆 丙泊酚 

分 类 号：R614[医药卫生—麻醉学]