rsa基因通过mbk-2调控秀丽隐杆线虫胚胎发育  

RSA gene regulates embryonic development of Caenorhabditis elegans by mbk-2

作　　者：翟佳佳[1] 张俊娣 左志洪[2] 王川 张雷 ZHAI Jia-jia;ZHANG Jun-di;ZUO Zhi-hong;WANG Chuan;ZHANG Lei(Department of Reproductive Medicine, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China;Department of Gynecology and Obstetrics, the People′s Hospital of Langfang, Hebei Province, Langfang 065000, China;Department of Pharmacology, the School of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China;Department of Histology and Embryology, the School of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China)

机构地区：[1]河北医科大学第二医院生殖医学科,河北石家庄050000 [2]河北省廊坊市人民医院妇产科,河北廊坊065000 [3]河北医科大学基础医学院药理学教研室,河北石家庄050017 [4]河北医科大学基础医学院组织胚胎学教研室,河北石家庄050017

出　　处：《河北医科大学学报》2020年第10期1154-1159,共6页Journal of Hebei Medical University

基　　金：国家自然科学基金资助项目(81770407);河北省自然科学基金资助项目(C2018206277)。

摘　　要：目的使用秀丽隐杆线虫作为模式生物,观察rsa基因对mbk-2突变型线虫胚胎发育和虫卵孵化的影响,初步探讨mbk-2的在胚胎发育中的作用及机制。方法以RNAi技术定向干扰mbk-2突变型线虫rsa基因的表达,并对rsa沉默后线虫的虫卵孵化水平及胚胎发育情况进行分析,检测其在线虫生长发育、生殖能力等方面的变化。结果与L4440喂养的mbk-2基因突变组相比,rsa-1和rsa-2RNAi细菌处理mbk-2基因突变组虫卵未孵化率显著升高,达到41.8%和49.2%(P<0.05),且rsa-2处理组显著高于rsa-1组(P<0.05);rsa-1和rsa-2 RNAi细菌处理的N2野生型线虫虫卵未孵化率分别为3.9%和5.7%,显著低于rsa-1和rsa-2 RNAi细菌处理的mbk-2突变型线虫(P<0.05)。rsa-2 RNAi处理mbk-2突变型线虫组,线虫胚胎发育异常显著增加。结论mbk-2是线虫发育的一个重要因子,rsa是mbk-2的重要修饰基因,两者相互作用调节线虫的胚胎发育和虫卵孵化。Objective To observe the effects of rsa gene on embryonic development and egg hatching of mbk-2 mutant C.elegans by using Caenorhabditis elegans(C.elegans)as a model organism,and the role and mechanism of mbk-2 in embryonic development were preliminarily discussed.Methods RNAi technology was used to directionally interfere with the expression of rsa gene in mbk-2 mutant C.elegans.The egg hatching level and embryonic development of nematode after rsa silencing were analyzed to detect the changes in the growth and reproductive capacity of C.elegans.Results Compared with mbk-2 gene mutation group fed with L4440,the hatchability of eggs in mbk-2 gene mutation group treated with rsa-1 and rsa-2 RNAi bacteria increased significantly,reaching 41.8%and 49.2%(P<0.05),and rsa-2 treatment group was significantly higher than rsa-1 group(P<0.05).The hatchability of eggs of N2 wild-type C.elegans treated by rsa-1 and rsa-2 RNAi bacteria was 3.9%and 5.7%respectively,which was significantly lower than that of mbk-2 mutant C.elegans treated by rsa-1 and rsa-2 RNAi bacteria(P<0.05).rsa-2 RNAi treatment of mbk-2 mutant C.elegans significantly increased abnormal embryonic development.Conclusion mbk-2 is an important factor in C.elegans development,rsa is an important modification gene of mbk-2,and the two interact to regulate C.elegans embryo development and egg hatching.

关 键 词：胚胎和胎儿发育 线虫纲 基因 

分 类 号：R714.51[医药卫生—妇产科学]