人诱导多功能干细胞分化的心肌细胞在心律失常建模中的应用  

Application of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Modeling of Arrhythmia

作　　者：郑泽群 廉姜芳[2] ZHENG Zequn;LIAN Jiangfang(Medical School of Ningbo University,Ningbo 315211,China;Lihuili Hospial Afliaed to Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学医学院,宁波315211 [2]宁波大学附属李惠利医院,宁波315211

出　　处：《中国细胞生物学学报》2020年第8期1457-1464,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金面上项目(批准号:81870255)资助的课题。

摘　　要：心律失常涉及多种心脏离子通道,理想的研究模型应能够表达各种通道,构成完整的动作电位,更好揭示心律失常复杂的机制。与成熟的人心脏电活动相比,异源表达体系或动物模型不可避免地存在较大差异。自体来源的人诱导多功能干细胞分化的心肌细胞(human-induced pluripotent stem cell-derived cardiomyocytes,hiPSC-CMs)建立的研究模型,在体外近乎完全地复制了心脏电生理活动特性,为疾病的研究搭建了广阔的平台。该文概述了以hiPSC-CMs为工具建立的心律失常模型,希望为相关研究提供一定的参考意义。Arrhythmia involves a variety of cardiac ion channels.The desirable research model should be able to characterize various channels that form a whole action potential,and then better reveal the complex mechanism under arrhythmia.Compared with mature human cardiac electrical activity,there are inevitably great differences in heterologous expression systems or animal models.The research models established by hiPSC-CMs (human-induced pluripotent stem cell-derived cardiomyocytes) derived from individuals have almost completely replicated the characteristics of cardiac electrophysiological activities in vitro,which build up a broad platform for the study of diseases.This article provides an overview of the arrhythmia models established with hiPSC-CMs,hoping to offer some references for related work.

关 键 词：人诱导多功能干细胞 人诱导多功能干细胞分化的心肌细胞 心律失常 疾病模型 

分 类 号：R541.7[医药卫生—心血管疾病]