Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato  

作　　者：WANG Zi-yu BAO Yu-fang PEI Tong WU Tai-ru DU Xu HE Meng-xi WANG Yue LIU Qi-feng YANG Huan-huan JIANG Jing-bin ZHANG He LI Jing-fu ZHAO Ting-ting XU Xiang-yang 

机构地区：[1]College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,P.R.China [2]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Harbin 150030,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第11期2699-2708,共10页农业科学学报（英文版）

基　　金：This research was supported by the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province,China(UNPYSCT-2018169);the China Postdoctoral Science Foundation Grant(2018 M630333);the National Key R&D Program of China(2017YFD0101900);the earmarked fund for China Agriculture Research System(CARS-23-A-16).

摘　　要：BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and development.SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress.In the present study,we used virus-induced gene silencing(VIGS)technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further.The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2 O2 and O2^–·,decreased superoxide dismutase(SOD)activity,and increased proline(PRO)and malondialdehyde(MDA)contents and peroxidase(POD)activity.Quantitative real-time PCR(qRT-PCR)analysis of BR-related genes revealed that the expression of SlCPD,SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress,but that the expression of TCH4-related genes was downregulated.These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.

关 键 词：SLB3 BES1 transcription factor VIGS drought gene expression BR signaling pathway 

分 类 号：S641.2[农业科学—蔬菜学]