Molecular diversity of barley yellow dwarf virus-PAV from China and the Czech Republic  

作　　者：May Oo KHINE Brozenka MICHAELA LIU Yan Jiban Kumar KUNDU WANG Xi-feng 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China [2]Division of Crop Protection and Plant Health,Crop Research Institute,Praha 16106,Czech Republic

出　　处：《Journal of Integrative Agriculture》2020年第11期2736-2745,共10页农业科学学报（英文版）

基　　金：This research was supported by the Inter-Governmental S&T Cooperation Project of China(2016YFE0131000);the Research Program of the Ministry of Education,Youth and Sports of the Czech Republic(LTACH-17010).

摘　　要：Wheat yellow dwarf disease(BYD),caused by different species of barley/cereal yellow dwarf viruses(B/CYDVs),is one of the most serious cereal diseases in China and the Czech Republic.Because genetic diversity of the virus directly influences disease epidemiology,the molecular diversity and population structure of 24 Chinese isolates and 16 the Czech Republic isolates of BYDV-PAV from different regions in two countries were analyzed by sequencing their coat protein(CP)and readthrough protein(RTP)domain(RTD)genes and comparing the sequences with six CP and 16 RTP sequences of BYDVPAV isolates from the NCBI database based on nucleotide identity position,phylogenetic analysis and nucleotide diversity.Nucleotide identities between the Chinese and the Czech Republic isolates for the CP were 76.6–99.4%,73.9–89.1%for RTD(ORF5),respectively.The Chinese and the other country isolates showed 74.7–99.2%nucleotide identity for RTP(ORF3+ORF5).Phylogenetic analysis of CP sequences showed that 20 Chinese isolates clustered in the same clade,but the other four Chinese isolates clustered in another clade with the isolates from the Czech Republic and other counties.The population of BYDV-PAV in China had greater nucleotide variability and was more divergent than that in the Czech Republic.Geographical and ecological factors but not hosts might contribute to the population differences in the two countries.

关 键 词：barley yellow dwarf virus-PAV coat protein(CP) readthrough protein(RTP) PHYLOGENY genetic diversity China the Czech Republic 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治]