Knockdown of the Meq gene in Marek’s disease tumor cell line MSB1 might induce cell apoptosis and inhibit cell proliferation and invasion  

作　　者：ZHAO Chun-fang LI Xin HAN Bo QU Lu-jiang LIU Chang-jun Jiu Zhou SONG YANG Ning LIAN Ling 

机构地区：[1]National Engineering Laboratory for Animal Breeding,Department of Animal Genetics and Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China [2]Department of Animal Science,Anhui Science and Technology University,Chuzhou 233100,P.R.China [3]Division of Avian Infectious Diseases,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,P.R.China [4]Department of Animal&Avian Sciences,University of Maryland,College Park,Maryland 20742,USA

出　　处：《Journal of Integrative Agriculture》2020年第11期2767-2774,共8页农业科学学报（英文版）

基　　金：The work was supported in part by the National Natural Science Foundation of China(31320103905 and U1901206);the Young Scientist Supporting Project,Program for Changjiang Scholars and Innovative Research Team in University(IRT_15R62);the earmarked fund for China Agriculture Research Systems(CARS-41);the National High Technology Development Plan of China(2013AA102501);the Farm Animals Germplasm Resource Bank;the Beijing Key Laboratory for Animal Genetic Improvement,the University Research Project of Anhui Province,China(KJ2020A0081);the Anhui Provincial Natural Science Foundation,China(2008085QC140);the Foundation of Anhui Science and Technology University,China(DKYJ201901);the Innovation Funds for Undergraduate Students of Anhui Province,China(S201910879019,S202010879109,and S202010879120).

摘　　要：Marek’s disease(MD),a highly cell-associated and contagious disease of chickens caused by Marek’s disease virus(MDV)can result in neural lesions,immunosuppression and neoplasia in chicken.The Meq gene is an important oncogene in the MDV genome,and it is expressed highly in MD tumor tissues and MD T-lymphoblastoid cell lines.An experiment was conducted to elucidate the role of Meq in MD tumor transformation.RNA interference technology was used to block its expression,and then analyzed the biological effects of Meq knockdown on the MD tumor cell line MSB1.A small interfering RNA with an interference efficiency of 70%(P<0.01)was transfected into MSB1 cells to knock down the expression of Meq gene.The cell proliferation,cycle and apoptosis were detected post-Meq knockdown.The results showed that MSB1 cell proliferation was downregulated remarkably at 48 h(P<0.01),60 h(P<0.05)and 72 h(P<0.01)post-Meq knockdown.The cell cycle was unaffected(P>0.05).B-cell lymphoma 2 gene(BCL2)was anti-apoptotic and caspase-6 was the effector in the apoptosis pathway.The activity of caspase-6 was upregulated(P<0.05)significantly and BCL2 gene expression was downregulated(P<0.05)significantly post-Meq knockdown,suggesting cell apoptosis might be induced.MSB1 cell migration did not exhibit any obvious change(P>0.05)post-Meq knockdown,but the expression of two genes(matrix metalloproteinase 2(MMP2)and MMP9)that are correlated closely to cell invasion was downregulated(P<0.05)remarkably post-Meq knockdown.The Meq knockdown might affect the main features of tumorous cells,including proliferation,apoptosis,and invasion,suggesting that the Meq gene might play a crucial role in interfering with lymphomatous cell transformation.

关 键 词：Marek’s disease MEQ proliferation migration INVASION 

分 类 号：S858.31[农业科学—临床兽医学]