罗哌卡因对新生大鼠脑细胞凋亡以及学习能力的影响  

Effects of ropivacaine on brain cell apoptosis and learning ability in neonatal mice

作　　者：李前辉[1] 谢小娟[1] 张宜林[1] Li Qianhui;Xie Xiaojuan;Zhang Yilin(Dept of Anesthesiology,The First Affiliated Hospital of Henan University of Science and Technology,Luoyang 471023)

机构地区：[1]河南科技大学第一附属医院麻醉科,洛阳471023

出　　处：《安徽医科大学学报》2020年第11期1710-1713,共4页Acta Universitatis Medicinalis Anhui

基　　金：河南省教育厅自然科学研究计划项目(编号:2015B20235)。

摘　　要：目的探讨罗哌卡因对新生大鼠脑细胞凋亡以及学习能力的影响。方法 60只出生10 d的新生大鼠随机分为对照组、低剂量组和高剂量组3组,每组20只,低剂量组和高剂量组采用微量注射器经腹腔分别给予0.5%的罗哌卡因20μl和60μl,对照组注射生理盐水60μl。3组大鼠在给药后12 h处死,分离血清采用ELISA测定血清炎症因子(TNF-α、IL-1β);分离脑组织,采用荧光定量PCR和Western blot分析炎症因子和凋亡相关蛋白的表达水平,采用TUNEL染色分析脑组织细胞凋亡情况。另取60只出生10 d的新生大鼠随机分对照组、低剂量组和高剂量组,每组20只,如上处理后29 d,采用Morris水平迷宫实验分析大鼠学习记忆能力。结果与对照组比较,低剂量组和高剂量组大鼠脑组织和血清炎症因子TNF-α和IL-1βmRNA和蛋白质水平显著增加,差异有统计学意义(P<0.05)。与对照组比较,低剂量组和高剂量组Bax和Caspase-3水平明显增加,Bcl-2水平显著下调,差异有统计学意义(P<0.05)。与对照组比较,低剂量组和高剂量组脑组织细胞凋亡水平明显增加,差异有统计学意义(P<0.05)。与低剂量组比较,高剂量组脑组织细胞凋亡水平增加更为显著,差异有统计学意义(P<0.05)。与对照组比较,在第29~32天,低剂量组和高剂量组大鼠逃避潜伏期明显延长,其中第31天和第32天,高剂量组逃避潜伏期高于低剂量组,差异有统计学意义(P<0.05)。结论罗哌卡因可引起新生大鼠脑组织出现炎症并伴有细胞凋亡,进而影响大鼠的学习记忆能力。Objective To investigate the effects of ropivacaine on brain cell apoptosis and learning ability in neonatal mice.Methods 60 newborn mice were randomly divided into three groups,control group:low dose group and high dose group(20 mices/group).Mice in low dose group and high dose group respectively by micro syringe was given intraperitoneally 0.5%ropivacaine 20μl and 60μl.Mice in the control group was injected with 60μl saline.6 mice in three groups after administration of 12 h were killed.Serum inflammatory factor serum(TNF-α,IL-1β)were analyzed by ELISA.The mRNA expression level of pro-inflammatory cytokines and apoptosis related protein was analyzed RT-PCR and Western blot.The apoptosis of cerebral tissue staining was analyzed by TUNEL.Another 60 old newborn rats were randomly divided into control group,low-dose group and high-dose group(20 mices/group).After treatment for 29 days,the ability of learning and memory of mice was analyzed by Morris level maze test.Results Compared with the control group,TNF-αand IL-1βmRNA and protein levels in the brain tissue and serum of the low dose group and high dose group significantly increased(P<0.05).Compared with the control group,the levels of Bax and Caspase-3 in the low-dose group and high-dose group significantly increased,and the level of Bcl-2 significantly decreased(P<0.05).Compared with the control group,the apoptosis of brain tissue in the low dose group and the high dose group increased significantly(P<0.05).Compared with the low dose group,the level of apoptosis in the high dose group increased more significantly(P<0.05).Compared with the control group,in 29-32 d,low dose group and high dose group mice escape latency was prolonged at 31 d and 32 d,the high dose group escape latency was higher than the low dose group(P<0.05).Conclusion Ropivacaine can induce inflammation and apoptosis in the brain tissue of neonatal mice,thereby affecting the learning and memory ability of mice.

关 键 词：罗哌卡因 炎症 凋亡 学习能力 

分 类 号：R971.2[医药卫生—药品]