子宫内膜间质细胞来源外泌体miRNA表达谱的差异分析  

Analysis of the difference of miRNA expression profile in endometrial stromal cell-derived exosomes

作　　者：王礼贤[1] 胡月阳 李琰[3] 杨漪[1] 康山 Wang Lixian;Hu Yueyang;Li Yan(Dept of Gynecology and Obsterics Ultrasonogrphy,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011;Laboratory Animal Center,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011;Dept of Molecular Biology,The Fourth Hospital of Hebei Medical University,Shijiazhuang 050011)

机构地区：[1]河北医科大学第四医院妇产超声科,石家庄050011 [2]河北医科大学第四医院实验动物中心,石家庄050011 [3]河北医科大学第四医院分子生物室,石家庄050011 [4]河北医科大学第四医院妇科,石家庄050011

出　　处：《安徽医科大学学报》2020年第11期1801-1805,共5页Acta Universitatis Medicinalis Anhui

基　　金：河北省医学科学研究重点课题计划项目(编号:20180506)。

摘　　要：探究不同来源子宫内膜间质细胞(ESC)外泌体微小RNA(miRNA)表达谱的差异。将40例子宫内膜异位症(EMs)患者(研究组)及40例子宫颈上皮内瘤变(CIN)Ⅲ行子宫手术患者(对照组)ESC原代培养后分离培养基中外泌体,芯片检测两组外泌体miRNA表达谱差异,qRT-PCR检测两组外泌体miR-205、miR-542-3p水平;对上调、下调倍数较高miRNA且与EMs相关靶基因进行预测。研究组与对照组ESC外泌体有39种miRNA表达差异有统计学意义(P<0.05),其中上调miRNA有14个,以miR-205上调倍数最高;下调有25个,以miR-542-3p下调倍数最高。qRT-PCR检测ESC外泌体miR-205、miR-542-3p水平与芯片检测倍数差异无统计学意义(P>0.05)。PTEN、FBN1、S100A2等与EMs相关的26种基因被预测为差异表达的miRNA靶基因。EMs患者ESC外泌体中存在多种差异表达的miRNA,且靶向多种EMs相关基因。To explore the difference of expression profiles of microRNA(miRNA)in the exosomes of endometrial stromal cells(ESC)from different sources.The exosomes were isolated from 40 patients with endometriosis(EMs)(study group)and 40 patients with cervical intraepithelial neoplasia(CIN)Ⅲunderwent uterine surgery(control group)after ESC primary culture.Chip was used to detect the difference of miRNA expression profile between the two groups.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the levels of miR-205 and miR-542-3 p in ESC exosomes.The target genes related to EMs of miRNA with higher up-regulated and down-regulated multiples were predicted.There were significant differences in the expression of 39 kinds of miRNA in ESC exosomes between the study group and the control group(P<0.05),among them,there were 14 up-regulated miRNA,with the up-regulated multiple of miR-205 was the highest;there were 25 down-regulated miRNA,with the down-regulated multiple of miR-542-3 p was the highest.qRT-PCR showed that there was no significant difference between the levels of miR-205 and miR-542-3 p in ESC exosomes with the chip detection multiples(P>0.05).26 genes related to EMs,such as PTEN,FBN1 and S100 A2,were predicted to be differentially expressed target genes of miRNA.There are many differentially expressed miRNA in ESC exosomes of EMs,targeting a variety of EMs-related genes.

关 键 词：子宫内膜异位症 子宫内膜间质细胞 外泌体 微小RNA 

分 类 号：R711.71[医药卫生—妇产科学] R737.33[医药卫生—临床医学]