硫化氢对大鼠基底动脉血管平滑肌细胞迁移、增殖及舒张作用的影响  

Effect of hydrogen sulfide on migration, proliferation and relaxation of rat basal artery vascular smooth muscle cells

作　　者：陈锦花 陈志武[1] Chen Jinhua;Chen Zhiwu(Dept of Pharmacologt,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学药理教研室,合肥230032

出　　处：《安徽医科大学学报》2020年第10期1510-1514,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金项目(编号:81374002、81973510)。

摘　　要：目的探讨硫化氢(H2S)对大鼠基底动脉(BAs)平滑肌细胞(VSMC)增殖、迁移及舒张的影响及其与血管内皮细胞生长因子受体2(VEGFR2)的关系。方法原代培养大鼠BAs VSMC。分别采用免疫荧光法鉴定BAs VSMC中VEGFR2表达,细胞划痕实验观察BAs VSMC迁移能力的改变,CCK-8法检测H2S对BAs VSMC增殖的影响,细胞舒张实验检测H2S对VSMC的舒张能力。结果免疫荧光检测结果显示VEGFR2在BAs VSMC中表达。细胞划痕和增值实验结果显示,与对照组比较,外源性H2S供体NaHS(100μmol/L)可提高BAs VSMC增殖、迁移能力,促进VSMC舒张(P<0.05)。与NaHS作用相似,10 ng/ml VEGF亦可增强BAs VSMC的迁移、增殖及舒张能力;而给予10-5 mol/L VEGFR2阻断剂SU5416预处理,可降低NaHS对细胞增殖、迁移及舒张。结论H2S可促进VSMC的增殖、迁移及细胞舒张,其作用可能与激动VEGFR2有关。Objective To investigate the effect of hydrogen sulfide(H2S) on proliferation, migration and relaxation of rat basilar artery(BAs) smooth muscle cells(VSMC) and the relationship between H2S and vascular endothelial growth factor receptor 2(VEGFR2). Methods Primary cultured rat BAs VSMC. Specifically, immunofluorescence was used to identify the expression of VEGFR2 in BAs VSMC. Cell scratch test was used to observe the change of migration ability of BAs VSMC. CCK-8 method was applied to detect the effect of H2S on the proliferation of BAs VSMC. Cell relaxation test was engaged to detect the relaxation ability of H2S on VSMC. Results The results of cell scratch and value-added experiments showed that, compared with the control group, exogenous H2S donor NaHS(100 μmol/L) could increase the proliferation and migration ability of BAs VSMC and promote VSMC relaxation(P<0.05). In addition, 10 ng/ml VEGF could also enhance the migration, proliferation and relaxation capacity of BAs VSMC, which was similar to the effect of NaHS. On the other hand, pretreatment with 10-5 mol/L VEGFR2 blocker SU5416 could reduce NaHS cell proliferation, migration and relaxation. Conclusion H2S can promote the proliferation, migration and cell relaxation of VSMC, which may be related to the stimulation of VEGFR2.

关 键 词：硫化氢 血管平滑肌细胞 血管内皮生长因子受体2 增殖 迁移 舒张 

分 类 号：R310.471[医药卫生—基础医学]