电针对肠易激综合征内脏痛大鼠β内啡肽及脑啡肽的调节作用  

Study on the Regulating Effects of Electroacupuncture onβ-Endorphin and Enkephalin in Visceral Pain Rats with Irritable Bowel Syndrome

作　　者：纪佳灏 黄作阵[1] JI Jiahao;HUANG Zuozhen(Beijing University of Traditional Chinese Meidicine,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029

出　　处：《世界中医药》2020年第19期2873-2877,共5页World Chinese Medicine

基　　金：北京中医药大学创新团队(2011-CXTD-06);北京市共建项目(0101186)。

摘　　要：目的:观察电针对肠易激综合征(IBS)内脏痛大鼠血清及下丘脑β-内啡肽(β-EP)及脑啡肽(ENK)的调节作用,探讨电针对IBS内脏痛的镇痛效应。方法:制备IBS内脏痛大鼠模型。实验分为模型组、电针组、假电针组和正常组。取天枢和上巨虚穴(双侧)为针刺穴位,针刺后连接电针仪;假电针组进针透皮即止,连接电针仪但不通电。模型组和正常组做与电针组相同的固定,不进行任何治疗。治疗后测试各组大鼠AWR评分,检测血清及下丘脑β-EP及ENK水平。结果:与正常组比较,模型组大鼠AWR评分在各个压力梯度下均显著升高、血清β-EP及ENK水平显著降低,差异有统计学意义(P<0.05),下丘脑β-EP、ENK水平差异无统计学意义(P>0.05)。与模型组、假电针组比较,电针组大鼠AWR评分明显降低,差异有统计学意义(P<0.05);血清和下丘脑β-EP、ENK水平显著增高,差异有统计学意义(P<0.05)。与模型组比较,假电针组大鼠AWR评分、血清和下丘脑β-EP、ENK水平差异无统计学意义(P>0.05)。结论:电针有效缓解IBS大鼠内脏痛,其作用机制可能与调节血清和下丘脑β-EP、ENK的水平有关。Objective:To observe the regulatory effects of electroacupuncture on serum and hypothalamicβ-endorphin(β-EP)and enkephalin(ENK)in rats with IBS visceral pain,and to explore the analgesic effect of electroacupuncture on IBS visceral pain.Methods:The rat model of IBS visceral pain was prepared.The experiment was divided into a model group,an electroacupuncture group,a sham electroacupuncture group and a normal group.Tianshu(S25)and Shangjuxu(S37)acupoints(both sides)was taken as acupuncture points,and the electroacupuncture instrument after acupuncture was connected.The sham electro-acupuncture group stopped when the needle was inserted through the skin,and the electro-acupuncture device was connected but not powered.The model group and the normal group received the same fixation as the electro-acupuncture group without any treatment.After treatment,the AWR scores of each group of rats were tested,and the serum and hypothalamicβ-EP and ENK contents were detected by ELISA.Results:Compared with the normal group,the AWR score of the model group was significantly increased under each pressure gradient,the serumβ-EP and ENK content were significantly reduced(all P<0.01),and the hypothalamicβ-EP and ENK content did not change significantly(P>0.05).Compared with the model group and the sham electroacupuncture group,the AWR score of the rats in the electroacupuncture group was significantly reduced(P<0.05);the serum and hypothalamicβ-EP and ENK levels were significantly increased(P<0.01,P<0.05).Compared with the model group,there were no significant changes in the AWR score,serum and hypothalamicβ-EP,ENK content of rats in the sham electroacupuncture group(P>0.05).Conclusion:Electroacupuncture can effectively relieve visceral pain in IBS rats,and its mechanism may be related to the regulation of the contents ofβ-EP and ENK in serum and hypothalamus.

关 键 词：电针 肠易激综合征 内脏痛 大鼠 β-内啡肽 脑啡肽 天枢 上巨虚 

分 类 号：R245[医药卫生—针灸推拿学]