穴位强化埋线疗法对STC患者直肠组织中ICC和神经元细胞的调节  

Regulation of ICC and Neuron Cells in Rectal Tissue of STC by Acupoint Intensive Catgut Embedding Therapy

作　　者：谢振年[1] 安晓静 杨斌[2] 李东冰[1] 王芳丽[3] 苗春红[4] 冷涛 贾小强[1] 赵卫兵[1] 曹威巍[1] 权隆芳[1] 原小千 崔春晖 XIE Zhennian;AN Xiaojing;YANG Bin;LI Dongbing;WANG Fangli;MIAO Chunhong;LENG Tao;JIA Xiaoqiang;ZHAO Weibing;CAO Weiwei;QUAN Longfang;YUAN Xiaoqian;CUI Chunhui(Department of Anorectal Surgery,Xiyuan Hospital,China Academy of Traditional Chinese Medicine,Beijing 100091,China;Department of Pathology,Xiyuan Hospital,Chinese Academy of Traditional Chinese Medicine,Beijing 100091,China;Department of General Surgery,Aerospace Center Hospital,Beijing 100083,China;Beijing Anorectal Hospital,Beijing,102401,China;Department of Anorectal Surgery,Pinggu District Hospital of Traditional Chinese Medicine,Beijing 101200,China)

机构地区：[1]中国中医科学院西苑医院肛肠外科,北京100091 [2]中国中医科学院西苑医院病理科,北京100091 [3]航天中心医院普外科,北京100083 [4]北京市肛肠医院,北京102401 [5]北京市平谷区中医院肛肠科,北京101200

出　　处：《世界中医药》2020年第19期2973-2977,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(81072813);第六批全国名老中医药专家学术经验继承工作资助项目;中国中医科学院西苑医院苗圃课题(2019XYMP-38)。

摘　　要：目的:探究穴位强化埋线疗法对慢传输型便秘(STC)直肠组织中ICC细胞和神经元的调节。方法:选择STC患者41例,非便秘患者41例,分别设置为观察组和对照组。观察组给予穴位强化埋线疗法,对照组未给予便秘相关治疗。比较观察组治疗前后直肠组织中ICC细胞形态、相关蛋白干细胞因子(STem Cell FacTor,SCF)和c-kit mRNA的表达水平和神经元凋亡情况。结果:观察组患者经穴位强化埋线治疗后直肠组织中ICC细胞的数量较治疗前增加,且形态趋于正常。与对照组比较,SCF和c-kit mRNA在治疗前直肠组织中的表达明显降低(P<0.05,P<0.01),而在治疗后直肠组织中的表达要高于治疗前(P<0.01)。治疗前,观察组患者直肠组织内神经元凋亡数量多于对照组(P<0.01)。治疗后患者直肠组织内凋亡细胞明显减少,与对照组比较差异有统计学意义(P<0.05)。结论:穴位强化埋线可改善STC患者直肠组织中ICC细胞的损伤,并减少神经元细胞的凋亡发生,从而改善便秘症状。Objective:To explore the regulation of acupoint intensive catgut embedding therapy on ICC cells and neurons in rectal tissue of slow transit constipation(STC).Methods:A total of 41 STC patients and 41 non-constipation patients were selected as the observation group and control group,respectively.The patients in treatment group were given acupoint intensive catgut embedding therapy,while the control group did not receive any constipation related treatment.The morphological of ICC and the expression of SCF and c-kit on RNA were compared before and after treatment in the treatment group.Results:The number of ICC in the rectum tissue increased with the normal morphology after treatment.Compared with the control group,both SCF mRNA and c-kit mRNA in the rectum were significantly lower without treatment(P<0.05,P<0.01).The expression in rectal tissue after treatment was higher than before treatment(P<0.01).Before treatment,the number of neuronal apoptosis in the rectal tissue of the observation group was more than that of the control group(P<0.01).After treatment,the apoptotic cells in the rectal tissue of the patients were significantly reduced,which was significantly different from the control group(P<0.05).Conclusion:Acupoint intensive catgut embedding therapy can improve the injury of ICC cells in rectal tissues of STC patients,reduce the apoptosis of neuron cells and improve the symptoms of constipation.

关 键 词：穴位强化埋线 慢传输型便秘 CAJAL间质细胞 干细胞因子 

分 类 号：R266[医药卫生—中医外科学] R245.91[医药卫生—中医学]