煅烧骨与羧甲基壳聚糖复合材料修复家兔下颌骨缺损的成骨效果  

Osteogenic Effect of Calcined Bone and Carboxymethyl Chitosan Composite Materials in Repairing Rabbit Mandibular Defect

作　　者：廖健 张霁文 程余婷 姚超 周倩 霍花 刘官娟 LIAO Jian;ZHANG Qiwen;CHEN Yuting;YAO Chao;ZHOU Qian;HUO Hua;LIU Guanjuan(Department of Prosthetics and Dental Implant,the Affiliated Stomatological Hospital of Guizhou Medical University,Guiyan 550004,Guizhou,China;School of Stomatology,Guizhou Medical University,Guiyan 550004,Guizhou,China;Guiyang Stomatological Hospital,Guiyang 550002,Guizhou,China)

机构地区：[1]贵州医科大学附属口腔医院修复种植科,贵州贵阳550004 [2]贵州医科大学口腔医学院,贵州贵阳550004 [3]贵阳市口腔医院,贵州贵阳550002

出　　处：《贵州医科大学学报》2020年第10期1156-1163,共8页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(81660179);贵州省自然科学基金[(2016)1124];贵州省自然科学基金联合基金[LH(2016)7257]。

摘　　要：目的:探讨煅烧骨/羧甲基壳聚糖(CB/CS)复合材料修复骨缺损的成骨效果。方法:制备家兔下颌骨缺损动物模型,分别将煅烧骨、Bio-oss骨粉、CB/CS复合材料、Bio-oss/羧甲基壳聚糖复合材料植入家兔下颌骨缺损处,并设不植入任何材料的空白对照组,每组12只;分别于术后第2、4、8及12周时处死家兔(每次每组各3只),取出术区标本观察骨缺损区大体结构变化,比较Micro-CT检测的骨密度(BMD)、骨小梁厚度或孔壁厚度(Tb.Th)、骨小梁分离度或孔隙率(Tb.Sp)及骨小梁数量(Tb.N)值评价成骨效果。结果:肉眼可见各时间段CB/CS组家兔的骨缺损愈合程度高于其其他各组家兔、新生骨质多于其他各组家兔,Micro-CT检测结果显示,术后第2、4、8及12周时各组BMD、Tb.Th、Tb.Sp及Tb.N值比较,差异有统计学意义(P<0.05),CB/CMC组在各时间段BMD、Tb.Th值均显著高于煅烧骨组、Bio-oss骨组及空白组,术后第2周开始CB/CMC组Tb.Sp值显著低于空白组和其他几组,术后第4、8、12周时CB/CMC组Tb.N值显著高于其他各组(P<0.05)。结论:CB/CS复合材料在植入动物体内后的12周内,能很好地修复骨缺损。Objective:To investigate osteogenic effect of calcined bone/carboxymethyl chitosan composite in repairing rabbit mandibular defect.Methods:The rabbit mandibular defect animal model was established.Calcined bone,Bio-oss bone meal,CB/CS composite material and Bio-oss/Carboxymethyl Chitosan composite material were implanted into the rabbit mandibular defect,while the blank control group was not implanted.At 2 nd,4 th,8 th and 12 th weeks after surgery,3 rabbits/group were sacrificed.The general structural changes of the bone defect areas in specimens of the operation areas and evaluated the osteogenesis effect via comparing BMD measured by Micro-CT,trabecular bone thickness or hole wall thickness(Tb.Th),trabecular bone separation or porosity(Tb.Sp)and number of trabecular bones(Tb.N)were observed.Results:CB/CS group had the best healing degree of bone defects and the most new bone mass.Micro-CT analysis revealed that BMD,Tb.Th and Tb.N of the Calcined bone/Carboxymethyl Chitosan composite material were significantly different at 2 nd,4 th,8 th and 12 th weeks after surgery(P<0.05).CB/CMC group had the highest BMD and Tb.Th values at 2 nd,4 th,8 th and 12 th weeks after surgery.Tb.Sp values in CB/CMC group were the lowest since 2 nd week after the surgery.In addition,Tb.N values of the CB/CMC group was the highest at 4 th,8 th and 12 th weeks after surgery(P<0.05).Conclusion:CB/CS composite materials can repair bone defects well within 12 weeks after implantation in animals.

关 键 词：煅烧骨 羧甲基壳聚糖 骨缺损 修复 显微-CT 

分 类 号：R318.08[医药卫生—生物医学工程] R782.6[医药卫生—基础医学]