Phylotranscriptomics in Cucurbitaceae Reveal Multiple Whole-Genome Duplications and Key Morphological and Molecular Innovations  

作　　者：Jing Guo Weibin Xu Yi Hu Jie Huang Yiyong Zhao Lin Zhang Chien-Hsun Huang Hong Ma 

机构地区：[1]Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and State Key Laboratory of Genetic Engineering,Institute of Biodiversity Sciences and Institute of Plant Biology,School of Life Sciences,Fudan University,2005 Songhu Road,Shanghai 200438,China [2]Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain,Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and Chinese Academy of Sciences,Guilin 541006,China [3]Department of Biology,the Huck Institutes of Life Sciences,the Pennsylvania State University,University Park,PA 16802,USA

出　　处：《Molecular Plant》2020年第8期1117-1133,共17页分子植物（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(grant no.31770242 and 31970224).

摘　　要：The ability of climbing plants to grow upward along others to reach the canopy for photosynthesis is hypothesized as a key innovation in flowering plants.Most members of the Cucurbitaceae,a family containing^1000 species and many important crops,are climbers and have characteristic tendrils and pepo fruits.Here,we present 127 newly sequenced transcdptomes and genomes along with other datasets for a total of 136 cucurbits representing all tribes to establish a robust Cucurbitaceae phylogeny containing eight highly resolved major clades.We analyzed whole-genome duplication,diversification dynamics,and ancestral morphologies,and found that after early genome duplication event(s),a burst of diversification and morphological innovations in flower,fruit,and root characters occurred under the climate optimum in the Early Eocene.Species radiation during the Mid-Eocene Climatic Optimum also coincided with several morphological changes shared by 80%of cucurbits.We found that the cucurbit-specific tendril identity gene TEN originated from a paleo-polyploidization event at the origin of the family.Our results support the hypothesis that cucurbit diversifications were probably driven by increased genetic diversity following polyploidizations and by trait morphological innovations under paleo-climate upheavals.Our study provides a phylogenetic framework and new insights into morphological and genomic changes underlying the adaptive evolution of Cucurbitaceae.

关 键 词：CUCURBITACEAE TRANSCRIPTOME PHYLOGENY divergence time genome duplication DIVERSIFICATION morphology adaptive evolution 

分 类 号：Q81[生物学—生物工程]