ESCRT-I Component VPS23A Sustains Salt Tolerance by Strengthening the SOS Module in Arabidopsis  

作　　者：Lijuan Lou Feifei Yu Miaomiao Tian Guangchao Liu Yaorong Wu Yujiao Wu Ran Xia Jose M.Pardo Yan Guo Qi Xie 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101 P.R.China [2]University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing 100193,P.R.China [4]Instituto de Bioquimica Vegetal y Fotosintesis,Consejo Superior de Investigaciones Cientificas and Universidad de Sevilla,Sevilla 41092,Spain

出　　处：《Molecular Plant》2020年第8期1134-1148,共15页分子植物（英文版）

基　　金：This project was financially supported by grants from the National Key R&D Program of China(2016YFA0500501);the National Natural Science Foundation of China(31800228 and 31571441);also partially supported by the Transgenic Research Projects(2016ZX08009-003).

摘　　要：The Salt-Overly-Sensitive(SOS)signaling module,comprising the sodium-transport protein SOS1 and the regulatory proteins SOS2 and SOS3,is well known as the central salt excretion system,which helps protect plants against salt stress.Here we report that VPS23A,a component of the ESCRT(endosomal sorting complex required for transport),plays an essential role in the function of the SOS module in conferring plant salt tolerance.VPS23A enhances the interaction of SOS2 and SOS3.In the presence of salt stress,VPS23A positively regulates the redistribution of SOS2 to the plasma membrane,which then activates the antiporter activity of SOS1 to reduce Na+accumulation in plant cells.Genetic evidence demonstrated that plant salt tolerance achieved by the overexpression of SOS2 and SOS3 dependeds on VPS23A.Taken together,our results revealed that VPS23A is a crucial regulator of the SOS module and affects the localization of SOS2 to the cell membrane.Moreover,the strong salt tolerance of Arabidopsis seedlings conferred by the engineered membrane-bound SOS2 revealed the significance of SOS2 sorting to the cell membrane in achieving its function,providing a potential strategy for crop salt tolerance engineering.

关 键 词：SOS pathway ESCRTs VPS23a SALT cell membrane 

分 类 号：Q94[生物学—植物学]