A Kinase-Phosphatase-Transcription Factor Module Regulates Adventitious Root Emergence in Arabidopsis Root-Hypocotyl Junctions  

作　　者：Zechen Bai Jing Zhang Xin Ning Hailong Guo Xiumei Xu Xiahe Huang Yingchun Wang Zhubing Hu Congming Lu Lixin Zhang Wei Chi 

机构地区：[1]Photosynthesis Research Center,Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Nanxincun 20,Xiangshan,Beijing 100093,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [4]State Key Laboratory of Crop Stress Adaptation and Improvement,School of Life Sciences,Henan University,85 Minglun Street,Kaifeng 475001,China [5]State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Taian,Shandong 271018,China [6]The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Molecular Plant》2020年第8期1162-1177,共16页分子植物（英文版）

基　　金：This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences

摘　　要：Adventitious roots form from non-root tissues as part of normal development or in response to stress or wounding.The root primordia form in the source tissue,and during emergence the adventitious roots penetrate the inner cell layers and the epidermis;however,the mechanisms underlying this emergence remain largely unexplored.Here,we report that a regulatory module composed of the AP2/ERF transcription factor ABSCISIC ACID INSENSITIVE 4(ABI4),the MAP kinases MPK3 and MPK6,and the phosphatase PP2C12 plays an important role in the emergence of junction adventitious roots(J-ARs)from the root-hypocotyl junctions in Arabidopsis thaliana.ABI4 negatively regulates J-AR emergence,preventing the accumulation of reactive oxygen species and death of epidermal cells,which would otherwise facilitate J-AR emergence.Phosphorylation by MPK3/MPK6 activates ABI4 and dephosphorylation by PP2C12 inactivates ABI4.MPK3/MPK6 also directly phosphorylate and inactivate PP2C12 during J-AR emergence.We propose that this"double-check"mechanism increases the robustness of MAP kinase signaling and finely regulates the local programmed cell death required for J-AR emergence.

关 键 词：ABI4 MPK3/MPK6 PP2C12 adventitious root root-hypocotyl junction PCD 

分 类 号：Q94[生物学—植物学]