Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology  

作　　者：Sadiqa FIRDOUS Samina IQBAL Samina ANWAR 

机构地区：[1]Soil and Environmental Biotechnology Division,National Institute for Biotechnology and Genetic Engineering(NIBGE),Faisalabad 38000(Pakistan) [2]Pakistan Institute of Engineering and Applied Sciences(PIEAS),Nilore,Islamabad 45650(Pakistan)

出　　处：《Pedosphere》2020年第5期618-627,共10页土壤圈（英文版）

基　　金：We acknowledge Higher Education Commission (HEC), Islamabad, Pakistan, for providing financial support through International Research Support Initiative Program (IRSIP) to conduct this research at University of Sydney, Australia.

摘　　要：Glyphosate is an important organophosphonate herbicide used to eliminate grasses and herbaceous plants in many vegetation management situations.Its extensive use is causing environmental pollution,and consequently,there is a need to remove it from the environment using an eco-friendly and cost-effective method.As a step to address this problem,a novel bacterial strain Comamonas odontotermitis F2,capable to utilize glyphosate as a carbon(C)and/or phosphorus(P)source,was isolated from a glyphostate-contaminated field soil in Australia and characterized.Response surface methodology(RSM)employing a 2^3 full factorial central composite design was used to optimize glyphosate degradation by C.odontotermitis P2 under various culture conditions.The strain C.odontotermitis P2 was proficient in degrading 1.5 g L^-1 glyphosate completely within 104 h.The optimal conditions for the degradation of glyphosate were found to be pH 7.4,29.9℃,and an inoculum density of 0.54 g L^-1,resulting in a maximum degradation of 90%.Sequencing of glyphosate oxidoreductase(GOX)and C-P lyase(phnJ)genes from C.odontotermitis P2 revealed 999c and 93%identities to already reported bacterial GOX and phnJ genes,respectively.The presence of these two genes in C.odontotermitis indicates its potential to degrade glyphosate through GOX and C-P lyase metabolic pathways.This study demonstrates the potential of C.odontotermitis P2 for efficient degradation of glyphosate,which can be exploited for remediation of glyphosate.

关 键 词：bacterial strain central composite rotatable design glyphosate oxidoreductase high-performance liquid chromatography inoculum density organophosphonate herbicide REMEDIATION 

分 类 号：S482.4[农业科学—农药学]