碱处理虫胶涂层的血液相容性和细胞活性研究  

Blood compatibility and cell viability of alkali treated shellac coatings

作　　者：任倩 覃利娜 景凤娟[1] 谢东[2] 黄楠[1] 冷永祥[1] REN Qian;QIN Lina;JING Fengjuan;XIE Dong;HUANG Nan;LENG Yongxiang(Key Laboratory for Advanced Technologies of Materials,Ministry of Education,School of Materials Science and Engineering,Southwest Jiao tong University,Chengdu 610031,China;School of Physics Science and Technology,Southwest Jiao tong University,Chengdu 610031,China)

机构地区：[1]西南交通大学材料科学与工程学院,材料先进技术教育部重点实验室,成都610031 [2]西南交通大学物理科学与技术学院,成都610031

出　　处：《功能材料》2020年第9期9126-9133,共8页Journal of Functional Materials

基　　金：四川省科技计划资助项目(2018HH0025)。

摘　　要：采用氢氧化钠溶液对虫胶进行碱处理后,采用旋涂法制备了具有较好结合力和血液相容性的虫胶涂层。采用傅里叶红外吸收光谱(FTIR)、X射线光电子能谱(XPS)、扫描电子显微镜(SEM)、水接触角仪(WCA)、多功能划痕仪等对涂层的表面结构、表面形貌和结合力等进行了分析,采用血小板粘附和溶血实验评价了涂层的血液相容性,采用CCK-8细胞计数盒研究了涂层的平滑肌细胞和内皮细胞的活性和增殖行为。研究结果显示,碱处理虫胶涂层与基体的结合力较好,不溶血,能够有效抑制血小板的粘附和激活,抑制平滑肌细胞的活性和增殖,并能一定程度地抑制内皮细胞的活性。碱处理过的虫胶涂层有望借鉴于心血管器械如静脉血栓过滤器的表面改性,以降低其取出时与组织粘连所产生的风险。The shellac was treated by sodium hydroxide and then spin-coated to prepare shellac coatings with good adhesion force and blood compatibility.The microstructure,surface morphology and adhesion force of the coatings were analyzed by Fourier infrared absorption spectrum(FTIR),X-ray photoelectron spectroscopy(XPS),scanning electron microscope(SEM),Water contact Angle meter(WCA),and scratching and bending tests.The platelet adhesion test and hemolysis assay were used to evaluate the blood compatibility of the coatings.The cell viability and proliferation of smooth muscle cells and endothelial cells were analyzed by CCK-8 assay.The results show that the adhesion force of the alkali-treated shellac coating was significantly improved than untreated shellac coatings.The alkali-treated shellac coatings could effectively inhibit platelet adhesion and activation compared to 316 L SS sample and showed low hemolysis rate of less than 5%,indicating blood combability of the coatings.The viability and proliferation of smooth muscle cells and endothelial cells of the alkali-treated shellac coatings were significantly suppressed compared to that of 316 L SS.Alkali-treated shellac coating was a potential material for surface modification of cardiovascular devices such as venous thrombus filters to reduce the risk of tissue adhesion.

关 键 词：虫胶涂层 碱处理 结合力 血小板 内皮细胞 平滑肌细胞 

分 类 号：TG430.55[金属学及工艺—焊接] R318.6[医药卫生—生物医学工程]