枯草芽孢杆菌生产β-丙氨酸的基因工程菌构建及其优化研究  

Construction and Optimization ofβ-Alanine Production by Bacillus Subtilis

作　　者：周洁静 Zhou Jie-jing(Chongzuo animal epidemic prevention and control center,Liaoning Chongzuo 532200)

机构地区：[1]崇左市动物疫病预防控制中心,辽宁崇左532200

出　　处：《生物化工》2020年第5期107-109,共3页Biological Chemical Engineering

摘　　要：将β-丙氨酸转入枯草芽孢杆菌,构建基因表达载体,优化了β-丙氨酸的密码子基因,构建出3种新型工程菌。通过比较工程菌的表达效果和可溶解性,优选出最适合菌种。Theβ-alanine was transferred into Bacillus subtilis to construct gene expression vector.The codon gene ofβ-alanine was optimized,and three new engineering bacteria were constructed.By comparing the expression effect and solubility of engineering bacteria,the most suitable strain was selected.

关 键 词：枯草芽孢杆菌 优化基因 目的蛋白 工程菌 Β-丙氨酸 

分 类 号：Q78[生物学—分子生物学]